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dc.contributor.authorMartins, Dulce Alvesen_UK
dc.contributor.authorRocha, Filipaen_UK
dc.contributor.authorMartinez-Rodriguez, Gonzaloen_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorMorais, Sofiaen_UK
dc.contributor.authorCastanheira, Filipaen_UK
dc.contributor.authorBandarra, Narcisaen_UK
dc.contributor.authorCoutinho, Joanaen_UK
dc.contributor.authorYufera, Manuelen_UK
dc.contributor.authorConceicao, Luis E Cen_UK
dc.description.abstractDietary fatty acid supply can affect stress response in fish during early development. Although knowledge on the mechanisms involved in fatty acid regulation of stress tolerance is scarce, it has often been hypothesised that eicosanoid profiles can influence cortisol production. Genomic cortisol actions are mediated by cytosolic receptors which may respond to cellular fatty acid signalling. An experiment was designed to test the effects of feeding gilthead sea-bream larvae with four microdiets, containing graded arachidonic acid (ARA) levels (0·4, 0·8, 1·5 and 3·0 %), on the expression of genes involved in stress response (steroidogenic acute regulatory protein, glucocorticoid receptor and phosphoenolpyruvate carboxykinase), lipid and, particularly, eicosanoid metabolism (hormone-sensitive lipase, PPARα, phospholipase A2, cyclo-oxygenase-2 and 5-lipoxygenase), as determined by real-time quantitative PCR. Fish fatty acid phenotypes reflected dietary fatty acid profiles. Growth performance, survival after acute stress and similar whole-body basal cortisol levels suggested that sea-bream larvae could tolerate a wide range of dietary ARA levels. Transcription of all genes analysed was significantly reduced at dietary ARA levels above 0·4 %. Nonetheless, despite practical suppression of phospholipase A2 transcription, higher leukotriene B4 levels were detected in larvae fed 3·0 % ARA, whereas a similar trend was observed regarding PGE2 production. The present study demonstrates that adaptation to a wide range of dietary ARA levels in gilthead sea-bream larvae involves the modulation of the expression of genes related to eicosanoid synthesis, lipid metabolism and stress response. The roles of ARA, other polyunsaturates and eicosanoids as signals in this process are discussed.en_UK
dc.publisherCambridge University Pressen_UK
dc.relationMartins DA, Rocha F, Martinez-Rodriguez G, Bell JG, Morais S, Castanheira F, Bandarra N, Coutinho J, Yufera M & Conceicao LEC (2012) Teleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genes. British Journal of Nutrition, 108 (5), pp. 864-874.
dc.rightsPublisher policy allows this work to be made available in this repository. Published in British Journal of Nutrition, Volume 108, Issue 05, September 2012, pp 864-874 copyright Cambridge University Press. The original publication is available at DOI:
dc.subjectSparus aurataen_UK
dc.subjectGene expressionen_UK
dc.titleTeleost fish larvae adapt to dietary arachidonic acid supply through modulation of the expression of lipid metabolism and stress response genesen_UK
dc.typeJournal Articleen_UK
dc.citation.jtitleBritish Journal of Nutritionen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Malagaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationINRB/IPIMAR, Portugalen_UK
dc.contributor.affiliationINRB/IPIMAR, Portugalen_UK
dc.contributor.affiliationSpanish National Research Council (CSIC)en_UK
dc.contributor.affiliationUniversity of Algarveen_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorMartins, Dulce Alves|en_UK
local.rioxx.authorRocha, Filipa|en_UK
local.rioxx.authorMartinez-Rodriguez, Gonzalo|en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorMorais, Sofia|en_UK
local.rioxx.authorCastanheira, Filipa|en_UK
local.rioxx.authorBandarra, Narcisa|en_UK
local.rioxx.authorCoutinho, Joana|en_UK
local.rioxx.authorYufera, Manuel|en_UK
local.rioxx.authorConceicao, Luis E C|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

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