Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/10491
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Effects of known phenoloxidase inhibitors on hemocyanin-derived phenoloxidase from Limulus polyphemus
Authors: Wright, Jamie
Clark, William McCaskill
Cairn, Jennifer A
Patterson, Alan
Coates, Christopher
Nairn, Jacqueline
Contact Email: jn2@stir.ac.uk
Keywords: Hemocyanin
Phenoloxidase
Hemocyanin-derived phenoloxidase
Kinetics
Inhibition
Issue Date: Nov-2012
Publisher: Elsevier
Citation: Wright J, Clark WM, Cairn JA, Patterson A, Coates C & Nairn J (2012) Effects of known phenoloxidase inhibitors on hemocyanin-derived phenoloxidase from Limulus polyphemus, Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 163 (3-4), pp. 303-308.
Abstract: Inhibitors of phenoloxidase are used routinely to characterise the structural and functional properties of phenoloxidases. Hemocyanin-derived phenoloxidase activity is also sensitive to standard phenoloxidase inhibitors. In this study, we characterise the effects of a number of phenoloxidase inhibitors on hemocyanin-derived phenoloxidase activity from the chelicerate, Limulus polyphemus. Both inhibition type and Ki values were similar to those observed for hemocyanin-derived phenoloxidase from another chelicerate, Eurypelma californicum. In addition, substrate inhibition was observed at concentrations above 2 mM dopamine. The conformation in which two of the inhibitors, namely tropolone and kojic acid, would bind near the Cu(II) centre of hemocyanin is proposed.
Type: Journal Article
URI: http://hdl.handle.net/1893/10491
DOI Link: http://dx.doi.org/10.1016/j.cbpb.2012.07.003
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.
Affiliation: University of Stirling
University of Stirling
University of Stirling
Campus Universidad Autonoma, Spain
Biological and Environmental Sciences
Biological and Environmental Sciences

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