Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9945
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dc.contributor.authorShinn, Andrew-
dc.contributor.authorGibson, David-
dc.contributor.authorSommerville, Christina-
dc.date.accessioned2012-11-15T23:13:14Z-
dc.date.issued1993-06-
dc.identifier.urihttp://hdl.handle.net/1893/9945-
dc.description.abstractTechniques for the preparation of haptoral sclerites of Gyrodactylus species for scanning electron microscopy are described. Enzymatic digestion using pepsin allows fresh, frozen and alcohol-fixed material to be examined, but some sclerites, such as the dorsal and ventral bars, are lost. Sonication of fresh and frozen material retained these accessory bars, including the ventral bar membrane, the guard or sickle filament loop on the marginal hooks and even on some occasions ligaments associated with the sclerites. The sclerites of Gyrodactylus spp. from Gasterosteus aculeatus (L.) (three-spined stickleback), Oncorhynchus mykiss (Walbaum) (rainbow trout) and Salvelinus alpinus (L.) (charr) are described from SEM observations following the use of these preparation techniques, and the articulation between the hamuli and bars is commented upon.en_UK
dc.language.isoen-
dc.publisherSpringer-
dc.relationShinn A, Gibson D & Sommerville C (1993) An SEM study of the haptoral sclerites of the genus Gyrodactylus Nordmann, 1832 (Monogenea) following extraction by digestion and sonication techniques, Systematic Parasitology, 25 (2), pp. 135-144.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.titleAn SEM study of the haptoral sclerites of the genus Gyrodactylus Nordmann, 1832 (Monogenea) following extraction by digestion and sonication techniquesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1007/BF00009983-
dc.citation.jtitleSystematic Parasitology-
dc.citation.issn0165-5752-
dc.citation.volume25-
dc.citation.issue2-
dc.citation.spage135-
dc.citation.epage144-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailaps1@stir.ac.uk-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationThe Natural History Museum-
dc.contributor.affiliationAquaculture-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
Appears in Collections:Aquaculture Journal Articles

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