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dc.contributor.authorCrockford, Tony-
dc.contributor.authorJohnston, Ian A-
dc.contributor.authorMcAndrew, Brendan-
dc.description.abstractSingle fast muscle fibres in the tropical fish Oreochromis andersonii were found to contain two myosin light chains (LC1s; LC1f1* or LC1f2*). Breeding experiments confirmed that the different LC1s were of allelic origin and their inheritance patterns conformed to Mendelian expectations (1:2:1). The LC1s differed in apparent relative molecular mass by 800-900. No other differences in myosin subunits were found between the LC1 genotypes. The molar ratios of LC3:LC1(total) in the fast muscle of O. andersonii homozygous for LC1f1* or LC1f2* and heterozygous for both alleles were 2.0:1, 2.1:1 and 2.2:1, respectively, as determined by capillary electrophoresis. The maximum contraction velocity (Vmax) of single skinned muscle fibres was determined at 20 °C by the slack-test method. Vmax values (fibre lengths s21) for fast muscle fibres from O. andersonii which were homozygous for either LC1f2* or LC1f1* were 5.3 and 3.3, respectively, compared with 3.8 when both alleles were present. Crosses between Oreochromis niloticus and O. andersonii produced F1 hybrids which were heterozygous for either LC1n/LC1f1* or LC1n/LC1f2*, where LC1n is the myosin light chain for O. niloticus. The distribution of myosin light chain genotypes in hybrid offspring was not significantly different from the expected Mendelian 1:1 ratio (47%: 53%). The Vmax (fibre lengths s21) of muscle fibres containing LC1f2* from hybrid Oreochromis was 4.3 compared with 3.1 for the LC1f1* genotype. The results are consistent with a functionally significant allelic variation in myosin LC1 in fast muscle fibres from O. andersonii which is also expressed in hybrid genotypes.en_UK
dc.publisherThe Company of Biologists Ltd-
dc.relationCrockford T, Johnston IA & McAndrew B (1995) Functionally significant allelic variation in myosin light chain composition in a tropical cichlid, Journal of Experimental Biology, 198 (12), pp. 2501-2508.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectmuscle contractionen_UK
dc.subjectallelic variationen_UK
dc.subjectOreochromis niloticusen_UK
dc.subjectOreochromis andersoniien_UK
dc.titleFunctionally significant allelic variation in myosin light chain composition in a tropical cichliden_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleJournal of Experimental Biology-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationUniversity of St Andrews-
dc.contributor.affiliationUniversity of St Andrews-
Appears in Collections:Aquaculture Journal Articles

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