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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species
Author(s): Gheyas, Almas A
Cairney, Margaret
Gilmour, Ann E
Sattar, M Abdus
Das, Topan K
McAndrew, Brendan
Penman, David
Taggart, John
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Keywords: cross-species amplification
genetic markers
microsatellite enrichment
silver carp
Issue Date: Sep-2006
Date Deposited: 23-Oct-2012
Citation: Gheyas AA, Cairney M, Gilmour AE, Sattar MA, Das TK, McAndrew B, Penman D & Taggart J (2006) Characterization of microsatellite loci in silver carp (Hypophthalmichthys molitrix), and cross-amplification in other cyprinid species. Molecular Ecology Notes, 6 (3), pp. 656-659.
Abstract: Captive populations of silver carp (Hypophthalmichthys molitrix), a major aquaculture species in Asia, would undoubtedly benefit from genetic monitoring and improvement programs. We report the isolation and preliminary characterization of 16 microsatellite loci derived from both conventional and microsatellite-enriched libraries. Inheritance studies confirmed the allelic nature of observed polymorphisms at all loci, while identifying null alleles at two loci. These loci, having varying degrees of polymorphism, should provide useful markers for applied genetic studies. A high degree of cross-amplification among 10 other cyprinid species suggests that these loci may have more widespread utility.
DOI Link: 10.1111/j.1471-8286.2006.01288.x
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