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|Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family
|Brown, R Cameron
|Brown RC, Tsalavouta M, Terzoglou V, Magoulas A & McAndrew B (2005) Additional microsatellites for Sparus aurata and cross-species amplification within the Sparidae family. Molecular Ecology Notes, 5 (3), pp. 605-607. https://doi.org/10.1111/j.1471-8286.2005.01007.x
|Six new microsatellite loci were isolated and characterized in 32 individuals from a farm population of gilthead seabream (Sparus aurata). Expected heterozygosity at all loci was high, ranging from 0.835 to 0.958 with between 10 and 27 alleles per locus. A multiplex polymerase chain reaction protocol was developed using four of the loci. Cross-species amplification of the loci was tested in six species of the Sparidae family and four loci were successfully amplified in two or more related species.
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