Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9293
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dc.contributor.authorMorais, Sofiaen_UK
dc.contributor.authorSilva, Tomeen_UK
dc.contributor.authorCordeiro, Odeteen_UK
dc.contributor.authorRodrigues, Pedroen_UK
dc.contributor.authorGuy, Derrick Ren_UK
dc.contributor.authorBron, Jamesen_UK
dc.contributor.authorTaggart, Johnen_UK
dc.contributor.authorBell, J Gordonen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.date.accessioned2018-04-26T00:30:04Z-
dc.date.available2018-04-26T00:30:04Z-
dc.date.issued2012-09en_UK
dc.identifier.other448en_UK
dc.identifier.urihttp://hdl.handle.net/1893/9293-
dc.description.abstractBackground: Expansion of aquaculture requires alternative feeds and breeding strategies to reduce dependency on fish oil (FO) and better utilization of dietary vegetable oil (VO). Despite the central role of intestine in maintaining body homeostasis and health, its molecular response to replacement of dietary FO by VO has been little investigated. This study employed transcriptomic and proteomic analyses to study effects of dietary VO in two family groups of Atlantic salmon selected for flesh lipid content, 'Lean' or 'Fat'. Results: Metabolism, particularly of lipid and energy, was the functional category most affected by diet. Important effects were also measured in ribosomal proteins and signalling. The long-chain polyunsaturated fatty acid (LC-PUFA) biosynthesis pathway, assessed by fatty acid composition and gene expression, was influenced by genotype. Intestinal tissue contents of docosahexaenoic acid were equivalent in Lean salmon fed either a FO or VO diet and expression of LC-PUFA biosynthesis genes was up-regulated in VO-fed fish in Fat salmon. Dietary VO increased lipogenesis in Lean fish, assessed by expression of FAS, while no effect was observed on β-oxidation although transcripts of the mitochondrial respiratory chain were down-regulated, suggesting less active energetic metabolism in fish fed VO. In contrast, dietary VO up-regulated genes and proteins involved in detoxification, antioxidant defence and apoptosis, which could be associated with higher levels of polycyclic aromatic hydrocarbons in this diet. Regarding genotype, the following pathways were identified as being differentially affected: proteasomal proteolysis, response to oxidative and cellular stress (xenobiotic and oxidant metabolism and heat shock proteins), apoptosis and structural proteins particularly associated with tissue contractile properties. Genotype effects were accentuated by dietary VO. Conclusions: Intestinal metabolism was affected by diet and genotype. Lean fish may have higher responsiveness to low dietary n-3 LC-PUFA, up-regulating the biosynthetic pathway when fed dietary VO. As global aquaculture searches for alternative oils for feeds, this study alerts to the potential of VO introducing contaminants and demonstrates the detoxifying role of intestine. Finally, data indicate genotype-specific responses in the intestinal transcriptome and proteome to dietary VO, including possibly structural properties of the intestinal layer and defence against cellular stress, with Lean fish being more susceptible to diet-induced oxidative stress.en_UK
dc.language.isoenen_UK
dc.publisherBioMed Centralen_UK
dc.relationMorais S, Silva T, Cordeiro O, Rodrigues P, Guy DR, Bron J, Taggart J, Bell JG & Tocher DR (2012) Effects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar). BMC Genomics, 13, Art. No.: 448. https://doi.org/10.1186/1471-2164-13-448en_UK
dc.rightsThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/2.0/en_UK
dc.subjectAtlantic salmonen_UK
dc.subjectgenotypeen_UK
dc.subjectnutritionen_UK
dc.subjectfish oilen_UK
dc.subjectvegetable oilen_UK
dc.subjectfaten_UK
dc.subjectleanen_UK
dc.subjectintestineen_UK
dc.subjecttransvcriptome.en_UK
dc.titleEffects of genotype and dietary fish oil replacement with vegetable oil on the intestinal transcriptome and proteome of Atlantic salmon (Salmo salar)en_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/1471-2164-13-448en_UK
dc.identifier.pmid22943471en_UK
dc.citation.jtitleBMC Genomicsen_UK
dc.citation.issn1471-2164en_UK
dc.citation.volume13en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationLandcatch Natural Selection Ltden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000309413700001en_UK
dc.identifier.scopusid2-s2.0-84865627969en_UK
dc.identifier.wtid889513en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2012-09-30en_UK
dc.date.filedepositdate2012-10-03en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorMorais, Sofia|en_UK
local.rioxx.authorSilva, Tome|en_UK
local.rioxx.authorCordeiro, Odete|en_UK
local.rioxx.authorRodrigues, Pedro|en_UK
local.rioxx.authorGuy, Derrick R|en_UK
local.rioxx.authorBron, James|0000-0003-3544-0519en_UK
local.rioxx.authorTaggart, John|0000-0002-3843-9663en_UK
local.rioxx.authorBell, J Gordon|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2012-10-03en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/2.0/|2012-10-03|en_UK
local.rioxx.filename1471-2164-13-448.pdfen_UK
local.rioxx.filecount1en_UK
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