Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/9292
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dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorGuinot, Dianaen_UK
dc.contributor.authorHontoria, Franciscoen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorNavarro, Juan Carlosen_UK
dc.date.accessioned2013-06-10T23:22:14Z-
dc.date.available2013-06-10T23:22:14Z-
dc.date.issued2012-09en_UK
dc.identifier.urihttp://hdl.handle.net/1893/9292-
dc.description.abstractPolyunsaturated fatty acids (PUFA) have been identified as key nutrients for the common octopus (Octopus vulgaris), particularly for its early life-cycle stages (paralarvae). Our overarching aim is to establish the essential fatty acid (FA) requirements for octopus paralarvae through determination of the enzymes of endogenous PUFA biosynthetic pathways. We here report on the molecular cloning and functional characterisation of a cDNA encoding a putative elongase of very long-chain fatty acids (Elovl), a critical enzyme that mediate the elongation of FA including PUFA. Our results suggested that the octopus Elovl is phylogenetically related to Elovl5 and Elovl2, two elongases with demonstrated roles in PUFA biosynthesis in vertebrates. Further evidence supporting a role of the octopus Elovl in PUFA biosynthesis was provided through functional characterisation of its activity in yeast. It was confirmed that expression of the octopus Elovl conferred on yeast the ability to elongate some C18 and C20 PUFA, while C22 PUFA substrates remained unmodified. The substrate specificities exhibited by the octopus elongase were consistent with those of vertebrate Elovl5. Interestingly, the octopus Elovl elongated n-6 PUFA substrates more efficiently than their analogous n-3 substrates, suggesting that n-6 PUFA may have particular biological significance in O. vulgaris. Finally, we investigated the potential role of the newly cloned Elovl in the biosynthesis of non-methylene-interrupted FA, compounds typically found in marine invertebrates and confirmed to be also present in the common octopus.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMonroig O, Guinot D, Hontoria F, Tocher DR & Navarro JC (2012) Biosynthesis of essential fatty acids in Octopus vulgaris (Cuvier, 1797): Molecular cloning, functional characterisation and tissue distribution of a fatty acyl elongase. Aquaculture, 360-361, pp. 45-53. https://doi.org/10.1016/j.aquaculture.2012.07.016en_UK
dc.rightsPublisher policy allows this work to be made available in this repository. Published in Aquaculture by Elsevier. The original publication is available at http://dx.doi.org/10.1016/j.aquaculture.2012.07.016en_UK
dc.subjectElongasesen_UK
dc.subjectessential fatty acidsen_UK
dc.subjectnon-methylene interrupted fatty acidsen_UK
dc.subjectOctopus vulgarisen_UK
dc.subjectpolyunsaturated fatty acids.en_UK
dc.subjectOmega-3 fatty acids Health aspects.en_UK
dc.titleBiosynthesis of essential fatty acids in Octopus vulgaris (Cuvier, 1797): Molecular cloning, functional characterisation and tissue distribution of a fatty acyl elongaseen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.aquaculture.2012.07.016en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume360-361en_UK
dc.citation.spage45en_UK
dc.citation.epage53en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.identifier.isiWOS:000308071400007en_UK
dc.identifier.scopusid2-s2.0-84864772449en_UK
dc.identifier.wtid757513en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2012-09-30en_UK
dc.date.filedepositdate2012-10-03en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorGuinot, Diana|en_UK
local.rioxx.authorHontoria, Francisco|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorNavarro, Juan Carlos|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2012-10-03en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2012-10-03|en_UK
local.rioxx.filenameOctopus Elongase Final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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