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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets
Author(s): Brunt, Jason
Hansen, Rasmus
Jamieson, Derek
Austin, Brian
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Keywords: Probiotic
Fish disease
Acute phase response
Innate immunity
Issue Date: 15-Feb-2008
Date Deposited: 31-Aug-2012
Citation: Brunt J, Hansen R, Jamieson D & Austin B (2008) Proteomic analysis of rainbow trout (Oncorhynchus mykiss, Walbaum) serum after administration of probiotics in diets. Veterinary Immunology and Immunopathology, 121 (3-4), pp. 199-205.
Abstract: The response of rainbowtrout (Oncorhynchusmykiss, Walbaum) towards probiotics present in the feed was investigated by examining the proteome of serum as a measure of the acute phase response (APR). Proteomicanalysis by two-dimensional electrophoresis (2D) concurrently with mass spectrometry was used to detect APR related proteins in rainbowtroutserum following feeding with probioticsAeromonas sobria GC2 and Bacillus sp. JB-1. Three candidate proteins increased following use of GC2, and were putatively identified as NADH dehydrogenase, dystrophin and mKIAA0350. Conversely, one of the proteins, which were induced following use of JB-1 was identified as transferrin.
DOI Link: 10.1016/j.vetimm.2007.09.010
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