Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7731
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dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorWang, Shuqien_UK
dc.contributor.authorZhang, Liangen_UK
dc.contributor.authorYou, Cuihongen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorLi, Yuanyouen_UK
dc.date.accessioned2013-06-09T03:30:19Z-
dc.date.available2013-06-09T03:30:19Z-
dc.date.issued2012-06en_UK
dc.identifier.urihttp://hdl.handle.net/1893/7731-
dc.description.abstractElongases of very long-chain fatty acids (Elovl) catalyse the rate-limiting step of the elongation pathway that results in net 2 C elongation of pre-existing fatty acyl chains. As the biosynthesis of long-chain polyunsaturated fatty acids (LC-PUFA) is particularly relevant in fish, Elovl involved in the pathway have been investigated in various studies. Here we report the molecular cloning, functional characterisation and tissue distribution of two distinct elovl-like cDNAs isolated from the herbivorous marine teleost Siganus canaliculatus. Unlike the carnivorous marine fish previously investigated, we hypothesise that the rabbitfish has an enhanced LC-PUFA biosynthetic capability as previously anticipated in a former study on fatty acyl desaturases (Fad). The results of the present study showed that rabbitfish expresses at least two elovl cDNAs, which have high homology in sequence and function to Elovl5 and Elovl4 elongases that have been investigated previously in other fish species. Furthermore, the results confirm that the activities of the Elovl5 and Elovl4 enzymes enable rabbitfish to perform all the elongation reactions required for the biosynthesis of the physiologically essential C20-22 LC-PUFA including eicosapentaenoic (20:5n-3), arachidonic (20:4n-6) and docosahexaenoic (22:6n-3, DHA) acids, as well as the less common very long-chain fatty acids (greater than C24). Rabbitfish is thus the first marine teleost in which genes encoding Fad and Elovl enzymes, with all the activities required for the production of DHA from C18 PUFA, have been characterised. Highlights Rabbitfish possess at least two fatty acid elongases. The elongases were functionally characterised as Elovl5 and Elovl4 types.  The elongases possess all the activities necessary for the biosynthesis of DHA.  Elovl4 is capable of synthesising very long-chain fatty acids up to C36 in length.  Rabbitfish represent a marine fish not dependent upon dietary EPA and DHAen_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationMonroig O, Wang S, Zhang L, You C, Tocher DR & Li Y (2012) Elongation of long-chain fatty acids in rabbitfish Siganus canaliculatus: Cloning, functional characterisation and tissue distribution of Elovl5- and Elovl4-like elongases. Aquaculture, 350-353, pp. 63-70. http://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84861039378&md5=56f7bdc22494e61a8a406ece49778ba4; https://doi.org/10.1016/j.aquaculture.2012.04.017en_UK
dc.rightsPublisher policy allows this work to be made available in this repository. Published in Aquaculture by Elsevier. The original publication is available at dx.doi.org/10.1016/j.aquaculture.2012.04.017en_UK
dc.subjectElovl4en_UK
dc.subjectElovl5en_UK
dc.subjectFatty acid biosynthesisen_UK
dc.subjectSiganus canaliculatusen_UK
dc.titleElongation of long-chain fatty acids in rabbitfish Siganus canaliculatus: Cloning, functional characterisation and tissue distribution of Elovl5- and Elovl4-like elongasesen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1016/j.aquaculture.2012.04.017en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume350-353en_UK
dc.citation.spage63en_UK
dc.citation.epage70en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.identifier.urlhttp://www.scopus.com/inward/record.url?partnerID=yv4JPVwI&eid=2-s2.0-84861039378&md5=56f7bdc22494e61a8a406ece49778ba4en_UK
dc.author.emaild.r.tocher@stir.ac.uken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationShantou Universityen_UK
dc.identifier.isiWOS:000305068800010en_UK
dc.identifier.scopusid2-s2.0-84861039378en_UK
dc.identifier.wtid772081en_UK
dc.contributor.orcid0000-0001-8712-0440en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dcterms.dateAccepted2012-06-30en_UK
dc.date.filedepositdate2012-08-29en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMonroig, Oscar|0000-0001-8712-0440en_UK
local.rioxx.authorWang, Shuqi|en_UK
local.rioxx.authorZhang, Liang|en_UK
local.rioxx.authorYou, Cuihong|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorLi, Yuanyou|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2012-08-29en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2012-08-29|en_UK
local.rioxx.filenameMonroig et al complete final.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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