Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7472
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dc.contributor.authorWilding, Craig Sen_UK
dc.contributor.authorTrikic, Michael Zen_UK
dc.contributor.authorHingston, Joanne Len_UK
dc.contributor.authorCopplestone, Daviden_UK
dc.contributor.authorTawn, E Janeten_UK
dc.date.accessioned2012-08-12T13:23:20Z-
dc.date.available2012-08-12T13:23:20Zen_UK
dc.date.issued2006-01-31en_UK
dc.identifier.urihttp://hdl.handle.net/1893/7472-
dc.description.abstractThe compost worm Eisenia fetida is routinely used in ecotoxicological studies. A standard assay to assess genetic damage in this species would be extremely valuable. Since mitochondrial DNA (mtDNA) is known to exhibit an increased mutation rate following exposure to ionising radiation we assessed the validity of a mtDNA-based assay for measuring increases in mutation rate in laboratory-irradiated compost worms. To this end the mutation frequency in the mtDNA of the compost worm E. fetida was quantified following in vivo γ-irradiation of adult worms in three dose groups. Five adult worms exposed to 1.4 mGy/h for 55 days (total dose 1.85 Gy), five adult worms exposed to 8.5 mGy/h for 55 days (total dose 11.22 Gy) and five adult control worms were used to assess the effect of irradiation on mtDNA mutation induction. DNA samples extracted from irradiated adult worms were used in high-fidelity PCR of a 486 bp region of mtDNA spanning the ATPase 8 gene, chosen for its high spontaneous mutation rate. PCR products were cloned and sequenced to identify mutations, with 89–102 clones successfully sequenced per individual. A significant elevation in mtDNA mutation frequency (p = 0.032) was seen in worms exposed at the higher dose rate (8.5 mGy/h, total dose 11.22 Gy; mutation frequency 27.98 ± 4.85 × 10−5 mutations/bp) in comparison to controls (mutation frequency 12.68 ± 3.06 × 10−5 mutations/bp), but no elevation in mutation frequency (p = 0.764) was seen for the lower dose rate (1.4 mGy/h, total dose 1.85 Gy; mutation frequency 13.74 ± 1.29 × 10−5 mutations/bp) compared with controls. This indicates that although the technique has the potential to detect an elevation in mutation frequency, it does not have sufficient sensitivity at the doses likely to be encountered in environmental monitoring scenarios.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationWilding CS, Trikic MZ, Hingston JL, Copplestone D & Tawn EJ (2006) Mitochondrial DNA mutation frequencies in experimentally irradiated compost worms, Eisenia fetida. Mutation Research - Genetic Toxicology and Environmental Mutagenesis, 603 (1), pp. 56-63. https://doi.org/10.1016/j.mrgentox.2005.10.011en_UK
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.rights.urihttp://www.rioxx.net/licenses/under-embargo-all-rights-reserveden_UK
dc.subjectanneliden_UK
dc.subjectbiomarkeren_UK
dc.subjectcompost wormen_UK
dc.subjectionising radiationen_UK
dc.subjectmitochondrial DNAen_UK
dc.subjectmutationen_UK
dc.titleMitochondrial DNA mutation frequencies in experimentally irradiated compost worms, Eisenia fetidaen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate3000-01-01en_UK
dc.rights.embargoreason[copplestone_mutationresearch_2006.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.identifier.doi10.1016/j.mrgentox.2005.10.011en_UK
dc.citation.jtitleMutation Research - Genetic Toxicology and Environmental Mutagenesisen_UK
dc.citation.issn1383-5718en_UK
dc.citation.volume603en_UK
dc.citation.issue1en_UK
dc.citation.spage56en_UK
dc.citation.epage63en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.author.emaildavid.copplestone@stir.ac.uken_UK
dc.contributor.affiliationWestlakes Scientific Consulting Ltden_UK
dc.contributor.affiliationWestlakes Scientific Consulting Ltden_UK
dc.contributor.affiliationUniversity of Liverpoolen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationWestlakes Scientific Consulting Ltden_UK
dc.identifier.isiWOS:000235272300007en_UK
dc.identifier.wtid779806en_UK
dc.contributor.orcid0000-0002-1468-9545en_UK
dcterms.dateAccepted2006-01-31en_UK
dc.date.filedepositdate2012-08-10en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorWilding, Craig S|en_UK
local.rioxx.authorTrikic, Michael Z|en_UK
local.rioxx.authorHingston, Joanne L|en_UK
local.rioxx.authorCopplestone, David|0000-0002-1468-9545en_UK
local.rioxx.authorTawn, E Janet|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate3000-01-01en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||en_UK
local.rioxx.filenamecopplestone_mutationresearch_2006.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1383-5718en_UK
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