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dc.contributor.authorWang, Yan-
dc.contributor.authorZhang, Xiao-Hua-
dc.contributor.authorAustin, Brian-
dc.description.abstractEdwardsiella tarda is a causative agent of edwardsiellosis in freshwater and marine fish. Extracellular enzymic, haemolytic, hydrophobic and serum resistance activities, haemagglutination, autoagglutination and siderophores of high- and low- virulent E. tarda strains were examined. The results revealed different haemagglutination, autoagglutination, haemolytic, hydrophobic and serum resistance activities in different strains. Analysis of extracellular proteins (ECPs) and outer membrane proteins (OMPs) demonstrated several major, low molecular weight, virulent-strain-specific proteins, which could be virulence- related. Based on the database search with MALDI-TOF MS data, the closest homologies of the three protein bands Ed1, Ed2 and Ed3 were phosphotransferase enzyme family protein, nitrite reductase [NAD(P)H], large subunit and ATP- dependent Lon protease, respectively. A comparison of pathogenicity of purified lipopolysaccharide (LPS) and lipid A from virulent and avirulent strains demonstrated that LPS was one of the virulence factors of the E. tarda isolates, and lipid A was a biologically active determinant of LPS.en_UK
dc.relationWang Y, Zhang X & Austin B (2010) Comparative analysis of the phenotypic characteristics of high- and low-virulent strains of Edwardsiella tarda, Journal of Fish Diseases, 33 (12), pp. 985-994.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectEdwardsiella tardaen_UK
dc.subjectlipid Aen_UK
dc.subjectSDS-polyacrylamide gel electrophoresisen_UK
dc.titleComparative analysis of the phenotypic characteristics of high- and low-virulent strains of Edwardsiella tardaen_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleJournal of Fish Diseases-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationHeriot-Watt University-
Appears in Collections:Aquaculture Journal Articles

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