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Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: PCR and molecular detection for differentiating Vibrio species
Author(s): Sparagano, Olivier A E
Robertson, Peter
Purdom, Ian
McInnes, J
Li, Yun
Yu, Dehua
Du, Zong-Jun
Xu, Huaishu
Austin, Brian
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Keywords: vibriosis
16S PCR reactions
23S PCR reactions
Vibrio species
Issue Date: Oct-2002
Date Deposited: 6-Aug-2012
Citation: Sparagano OAE, Robertson P, Purdom I, McInnes J, Li Y, Yu D, Du Z, Xu H & Austin B (2002) PCR and molecular detection for differentiating Vibrio species. Annals of the New York Academy of Sciences, 969 (1), pp. 60-65.
Abstract: Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.
DOI Link: 10.1111/j.1749-6632.2002.tb04351.x
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