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http://hdl.handle.net/1893/7303
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DC Field | Value | Language |
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dc.contributor.author | Rasch, Maria | - |
dc.contributor.author | Buch, Christiane | - |
dc.contributor.author | Austin, Brian | - |
dc.contributor.author | Slierendrecht, Wilhelmina J | - |
dc.contributor.author | Ekmann, Kim S | - |
dc.contributor.author | Larsen, Jens | - |
dc.contributor.author | Johansen, Charlotte | - |
dc.contributor.author | Riedel, Kathrin | - |
dc.contributor.author | Eberl, Leo | - |
dc.contributor.author | Givskov, Michael | - |
dc.contributor.author | Gram, Lone | - |
dc.date.accessioned | 2018-02-01T09:49:40Z | - |
dc.date.issued | 2004-05 | - |
dc.identifier.uri | http://hdl.handle.net/1893/7303 | - |
dc.description.abstract | The fish pathogen Vibrio anguillarum produces quorum sensing signal molecules, N-acyl homoserine lactones (AHLs), which in several Gram-negative human and plant pathogenic bacteria regulate virulence factors. Expression of these factors can be blocked using specific quorum-sensing inhibitors (QSIs). The purpose of this study was to investigate the effect of a QSI, furanone C-30, on mortality of rainbow trout during challenge with V. anguillarum. Addition of 0.01 or 0.1 μM furanone C-30 to rainbow trout infected by cohabitation caused a significant reduction in accumulated mortality from 80-100% in challenge controls to 4-40% in treated groups. Furanone C-30 had no effect in an immersion challenge system, probably due to a very high water exchange and a rapid dilution of furanone C-30. Growth and survival of V. anguillarum were not affected by the concentrations of furanone C-30 used in the challenge experiments, thus avoiding selection for resistance. To elucidate the mechanism of disease control by furanone C-30, we determined its effect on the bacterial proteome, motility, and respiration. No effects were seen of furanone C-30 in any of these experiments. Although no cytotoxic effect on HeLa cells were observed, exposure to 1 micrometer (or higher) concentrations of furanone C-30 had detrimental effects on the rainbow trout. Our results indicate that QSIs can be used in non-antibiotic based control of fish diseases. However, they also underline the need for development of novel, less toxic QSI compounds and the need for understanding the exact mechanism(s) of action. | en_UK |
dc.language.iso | en | - |
dc.publisher | Elsevier | - |
dc.relation | Rasch M, Buch C, Austin B, Slierendrecht WJ, Ekmann KS, Larsen J, Johansen C, Riedel K, Eberl L, Givskov M & Gram L (2004) An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum), Systematic and Applied Microbiology, 27 (3), pp. 350-359. | - |
dc.rights | The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. | - |
dc.subject | Acylated homoserine lactones | en_UK |
dc.subject | Vibrio anguillarum | en_UK |
dc.subject | Quorum-sensing inhibitor | en_UK |
dc.subject | Furanone C-30 | en_UK |
dc.title | An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum) | en_UK |
dc.type | Journal Article | en_UK |
dc.rights.embargodate | 2999-12-31T00:00:00Z | - |
dc.rights.embargoreason | The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work. | - |
dc.identifier.doi | http://dx.doi.org/10.1078/0723-2020-00268 | - |
dc.citation.jtitle | Systematic and Applied Microbiology | - |
dc.citation.issn | 0723-2020 | - |
dc.citation.volume | 27 | - |
dc.citation.issue | 3 | - |
dc.citation.spage | 350 | - |
dc.citation.epage | 359 | - |
dc.citation.publicationstatus | Published | - |
dc.citation.peerreviewed | Refereed | - |
dc.type.status | Publisher version (final published refereed version) | - |
dc.author.email | brian.austin@stir.ac.uk | - |
dc.contributor.affiliation | Technical University of Denmark | - |
dc.contributor.affiliation | Technical University of Denmark | - |
dc.contributor.affiliation | Aquaculture | - |
dc.contributor.affiliation | BioMar AS | - |
dc.contributor.affiliation | BioMar AS | - |
dc.contributor.affiliation | Royal Veterinary and Agricultural University, Denmark | - |
dc.contributor.affiliation | Pantheco A/S, Denmark | - |
dc.contributor.affiliation | Technical University of Munich | - |
dc.contributor.affiliation | Technische Universitat Munchen, Germany | - |
dc.contributor.affiliation | Technical University of Denmark | - |
dc.contributor.affiliation | Technical University of Denmark | - |
dc.rights.embargoterms | 2999-12-31 | - |
dc.rights.embargoliftdate | 2999-12-31 | - |
dc.identifier.isi | 000222032100011 | - |
Appears in Collections: | Aquaculture Journal Articles |
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Austin_2004_An_Inhibitor_of_Bacterial_Quorum_Sensing.pdf | 220.49 kB | Adobe PDF | Under Permanent Embargo Request a copy |
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