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dc.contributor.authorRasch, Maria-
dc.contributor.authorBuch, Christiane-
dc.contributor.authorAustin, Brian-
dc.contributor.authorSlierendrecht, Wilhelmina J-
dc.contributor.authorEkmann, Kim S-
dc.contributor.authorLarsen, Jens-
dc.contributor.authorJohansen, Charlotte-
dc.contributor.authorRiedel, Kathrin-
dc.contributor.authorEberl, Leo-
dc.contributor.authorGivskov, Michael-
dc.contributor.authorGram, Lone-
dc.description.abstractThe fish pathogen Vibrio anguillarum produces quorum sensing signal molecules, N-acyl homoserine lactones (AHLs), which in several Gram-negative human and plant pathogenic bacteria regulate virulence factors. Expression of these factors can be blocked using specific quorum-sensing inhibitors (QSIs). The purpose of this study was to investigate the effect of a QSI, furanone C-30, on mortality of rainbow trout during challenge with V. anguillarum. Addition of 0.01 or 0.1 μM furanone C-30 to rainbow trout infected by cohabitation caused a significant reduction in accumulated mortality from 80-100% in challenge controls to 4-40% in treated groups. Furanone C-30 had no effect in an immersion challenge system, probably due to a very high water exchange and a rapid dilution of furanone C-30. Growth and survival of V. anguillarum were not affected by the concentrations of furanone C-30 used in the challenge experiments, thus avoiding selection for resistance. To elucidate the mechanism of disease control by furanone C-30, we determined its effect on the bacterial proteome, motility, and respiration. No effects were seen of furanone C-30 in any of these experiments. Although no cytotoxic effect on HeLa cells were observed, exposure to 1 micrometer (or higher) concentrations of furanone C-30 had detrimental effects on the rainbow trout. Our results indicate that QSIs can be used in non-antibiotic based control of fish diseases. However, they also underline the need for development of novel, less toxic QSI compounds and the need for understanding the exact mechanism(s) of action.en_UK
dc.relationRasch M, Buch C, Austin B, Slierendrecht WJ, Ekmann KS, Larsen J, Johansen C, Riedel K, Eberl L, Givskov M & Gram L (2004) An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum), Systematic and Applied Microbiology, 27 (3), pp. 350-359.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectAcylated homoserine lactonesen_UK
dc.subjectVibrio anguillarumen_UK
dc.subjectQuorum-sensing inhibitoren_UK
dc.subjectFuranone C-30en_UK
dc.titleAn inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleSystematic and Applied Microbiology-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationTechnical University of Denmark-
dc.contributor.affiliationTechnical University of Denmark-
dc.contributor.affiliationBioMar AS-
dc.contributor.affiliationBioMar AS-
dc.contributor.affiliationRoyal Veterinary and Agricultural University, Denmark-
dc.contributor.affiliationPantheco A/S, Denmark-
dc.contributor.affiliationTechnical University of Munich-
dc.contributor.affiliationTechnische Universitat Munchen, Germany-
dc.contributor.affiliationTechnical University of Denmark-
dc.contributor.affiliationTechnical University of Denmark-
Appears in Collections:Aquaculture Journal Articles

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