Please use this identifier to cite or link to this item:
Full metadata record
DC FieldValueLanguage
dc.contributor.authorRasch, Mariaen_UK
dc.contributor.authorBuch, Christianeen_UK
dc.contributor.authorAustin, Brianen_UK
dc.contributor.authorSlierendrecht, Wilhelmina Jen_UK
dc.contributor.authorEkmann, Kim Sen_UK
dc.contributor.authorLarsen, Jensen_UK
dc.contributor.authorJohansen, Charlotteen_UK
dc.contributor.authorRiedel, Kathrinen_UK
dc.contributor.authorEberl, Leoen_UK
dc.contributor.authorGivskov, Michaelen_UK
dc.contributor.authorGram, Loneen_UK
dc.description.abstractThe fish pathogen Vibrio anguillarum produces quorum sensing signal molecules, N-acyl homoserine lactones (AHLs), which in several Gram-negative human and plant pathogenic bacteria regulate virulence factors. Expression of these factors can be blocked using specific quorum-sensing inhibitors (QSIs). The purpose of this study was to investigate the effect of a QSI, furanone C-30, on mortality of rainbow trout during challenge with V. anguillarum. Addition of 0.01 or 0.1 μM furanone C-30 to rainbow trout infected by cohabitation caused a significant reduction in accumulated mortality from 80-100% in challenge controls to 4-40% in treated groups. Furanone C-30 had no effect in an immersion challenge system, probably due to a very high water exchange and a rapid dilution of furanone C-30. Growth and survival of V. anguillarum were not affected by the concentrations of furanone C-30 used in the challenge experiments, thus avoiding selection for resistance. To elucidate the mechanism of disease control by furanone C-30, we determined its effect on the bacterial proteome, motility, and respiration. No effects were seen of furanone C-30 in any of these experiments. Although no cytotoxic effect on HeLa cells were observed, exposure to 1 micrometer (or higher) concentrations of furanone C-30 had detrimental effects on the rainbow trout. Our results indicate that QSIs can be used in non-antibiotic based control of fish diseases. However, they also underline the need for development of novel, less toxic QSI compounds and the need for understanding the exact mechanism(s) of action.en_UK
dc.relationRasch M, Buch C, Austin B, Slierendrecht WJ, Ekmann KS, Larsen J, Johansen C, Riedel K, Eberl L, Givskov M & Gram L (2004) An inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum). Systematic and Applied Microbiology, 27 (3), pp. 350-359.
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.en_UK
dc.subjectAcylated homoserine lactonesen_UK
dc.subjectVibrio anguillarumen_UK
dc.subjectQuorum-sensing inhibitoren_UK
dc.subjectFuranone C-30en_UK
dc.titleAn inhibitor of bacterial quorum sensing reduces mortalities caused by vibriosis in rainbow trout (Oncorhynchus mykiss, Walbaum)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreason[Austin_2004_An_Inhibitor_of_Bacterial_Quorum_Sensing.pdf] The publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.en_UK
dc.citation.jtitleSystematic and Applied Microbiologyen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationBioMar ASen_UK
dc.contributor.affiliationBioMar ASen_UK
dc.contributor.affiliationRoyal Veterinary and Agricultural University, Denmarken_UK
dc.contributor.affiliationPantheco A/S, Denmarken_UK
dc.contributor.affiliationTechnical University of Munichen_UK
dc.contributor.affiliationTechnische Universitat Munchen, Germanyen_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
dc.contributor.affiliationTechnical University of Denmarken_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorRasch, Maria|en_UK
local.rioxx.authorBuch, Christiane|en_UK
local.rioxx.authorAustin, Brian|en_UK
local.rioxx.authorSlierendrecht, Wilhelmina J|en_UK
local.rioxx.authorEkmann, Kim S|en_UK
local.rioxx.authorLarsen, Jens|en_UK
local.rioxx.authorJohansen, Charlotte|en_UK
local.rioxx.authorRiedel, Kathrin|en_UK
local.rioxx.authorEberl, Leo|en_UK
local.rioxx.authorGivskov, Michael|en_UK
local.rioxx.authorGram, Lone|en_UK
local.rioxx.projectInternal Project|University of Stirling|
Appears in Collections:Aquaculture Journal Articles

Files in This Item:
File Description SizeFormat 
Austin_2004_An_Inhibitor_of_Bacterial_Quorum_Sensing.pdfFulltext - Published Version220.49 kBAdobe PDFUnder Embargo until 3000-01-01    Request a copy

This item is protected by original copyright

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved

If you believe that any material held in STORRE infringes copyright, please contact providing details and we will remove the Work from public display in STORRE and investigate your claim.