Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7212
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dc.contributor.authorPang, Long-
dc.contributor.authorZhang, Xiao-Hua-
dc.contributor.authorZhong, Yingbin-
dc.contributor.authorChen, Jixiang-
dc.contributor.authorLi, Yun-
dc.contributor.authorAustin, Brian-
dc.date.accessioned2012-08-07T13:03:23Z-
dc.date.issued2006-09-
dc.identifier.urihttp://hdl.handle.net/1893/7212-
dc.description.abstractAims: The aim of this study was to develop an effective method for the identification of Vibrio harveyi based on using the toxR gene as a taxonomic marker. Methods and Results: Primers for the toxR gene were designed for specificity to V. harveyi, and incorporated in a polymerase chain reaction (PCR). The results of the PCR, which took <5 h from DNA extraction to amplification, revealed positive amplification of the toxR gene fragment in 20 V. harveyi isolates including type strains, whereas DNA from 23 other Vibrionaceae type strains and 13 Vibrio parahaemolyticus strains were negative. The detection limit of the PCR was 4.0 × 103 cells ml−1. In addition, the technique enabled the recognition of V. harveyi from diseased fish. Conclusions: The PCR was specific and sensitive, enabling the identification of V. harveyi within 5 h. Significance and Impact of the Study: The PCR allowed the rapid and sensitive detection of V. harveyi.<en_UK
dc.language.isoen-
dc.publisherWiley-Blackwell-
dc.relationPang L, Zhang X, Zhong Y, Chen J, Li Y & Austin B (2006) Identification of Vibrio harveyi using PCR amplification of the toxR gene, Letters in Applied Microbiology, 43 (3), pp. 249-255.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectdiseased fishen_UK
dc.subjectidentificationen_UK
dc.subjectpolymerase chain reactionen_UK
dc.subjecttoxR geneen_UK
dc.subjectVibrio harveyien_UK
dc.titleIdentification of Vibrio harveyi using PCR amplification of the toxR geneen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2999-12-31T00:00:00Z-
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.identifier.doihttp://dx.doi.org/10.1111/j.1472-765X.2006.01962.x-
dc.citation.jtitleLetters in Applied Microbiology-
dc.citation.issn0266-8254-
dc.citation.volume43-
dc.citation.issue3-
dc.citation.spage249-
dc.citation.epage255-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailbrian.austin@stir.ac.uk-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationOcean University of China-
dc.contributor.affiliationHeriot-Watt University-
dc.rights.embargoterms2999-12-31-
dc.rights.embargoliftdate2999-12-31-
dc.identifier.isi000239702200003-
Appears in Collections:Aquaculture Journal Articles

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