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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Cryptic species identification: a simple diagnostic tool for discriminating between two problematic bumblebee species
Author(s): Ellis, Jonathan
Knight, Mairi E
Carvell, Claire
Goulson, Dave
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Keywords: Bombus
cryptic species
restriction enzyme
Issue Date: Jun-2006
Date Deposited: 3-Aug-2012
Citation: Ellis J, Knight ME, Carvell C & Goulson D (2006) Cryptic species identification: a simple diagnostic tool for discriminating between two problematic bumblebee species. Molecular Ecology Notes, 6 (2), pp. 540-542.
Abstract: Distinguishing between cryptic species is a perennial problem for biologists. Bombus ruderatus and Bombus hortorum are two species of bumblebee, which can be indistinguishable from their morphology. The former species is in decline, whereas the latter is ubiquitous. In the UK, isolated records of B. ruderatus occur amongst many for B. hortorum. For ecological studies of B. ruderatus to be feasible, the two species need to be reliably distinguishable. We present a diagnostic tool for quick and reliable identification of problematic individuals based on a restriction enzyme digest of the cytochrome b region of mitochondrial DNA.
DOI Link: 10.1111/j.1471-8286.2006.01231.x
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