Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/7174
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dc.contributor.authorBekaert, Michaël-
dc.contributor.authorTeeling, Emma C-
dc.date.accessioned2014-09-05T23:44:25Z-
dc.date.available2014-09-05T23:44:25Z-
dc.date.issued2008-06-
dc.identifier.urihttp://hdl.handle.net/1893/7174-
dc.description.abstractUniPrime is an open-source software ( http://uniprime.batlab.eu), which automatically designs large sets of universal primers by simply inputting a gene ID reference. UniPrime automatically retrieves and aligns homologous sequences from GenBank, identifies regions of conservation within the alignment and generates suitable primers that can amplify variable genomic regions. UniPrime differs from previous automatic primer design programs in that all steps of primer design are automated, saved and are phylogenetically limited. We have experimentally verified the efficiency and success of this program by amplifying and sequencing four diverse genes (AOF2, EFEMP1, LRP6 and OAZ1) across multiple Orders of mammals. UniPrime is an experimentally validated, fully automated program that generates successful cross-species primers that take into account the biological aspects of the PCR.en_UK
dc.language.isoen-
dc.publisherOxford University Press-
dc.relationBekaert M & Teeling EC (2008) UniPrime: a workflow-based platform for improved universal primer design, Nucleic Acids Research, 36 (10), p. e56.-
dc.rightsPublisher policy allows this work to be made available in this repository. Published in Nucleic Acids Research by Oxford University Press. Nucleic Acids Research (2008) 36 (10): e56. http://nar.oxfordjournals.org/content/36/10/e56-
dc.titleUniPrime: a workflow-based platform for improved universal primer designen_UK
dc.typeJournal Articleen_UK
dc.identifier.doihttp://dx.doi.org/10.1093/nar/gkn191-
dc.citation.jtitleNucleic Acids Research-
dc.citation.issn0305-1048-
dc.citation.volume36-
dc.citation.issue10-
dc.citation.spagee56-
dc.citation.publicationstatusPublished-
dc.citation.peerreviewedRefereed-
dc.type.statusPublisher version (final published refereed version)-
dc.author.emailmichael.bekaert@stir.ac.uk-
dc.contributor.affiliationAquaculture-
dc.contributor.affiliationUniversity College Dublin (UCD)-
dc.identifier.isi000257183200035-
Appears in Collections:Aquaculture Journal Articles

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