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dc.contributor.authorAbbass, Amany A-
dc.contributor.authorSharifuzzaman, S M-
dc.contributor.authorAustin, Brian-
dc.description.abstractSubcellular components of the probiotics Aeromonas sobria GC2 and Bacillus subtilis JB-1, when administered to rainbow trout, Oncorhynchus mykiss, conferred protection against a new biogroup of Yersinia ruckeri. Thus, intraperitoneal or intramuscular injection of rainbow trout with cell wall proteins (CWPs), outer membrane proteins (OMPs), lipopolysaccharides (LPS), whole cell proteins (WCPs) and live cells followed by challenge on day 8 with Y. ruckeri led to 80-100% survival compared with 10% survival in the controls. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) profiles of WCPs and OMPs from GC2 had 10 and 5 variable protein bands in comparison to 11 and 5 bands in the WCPs and CWPs from JB-1. Proteomic analyses were employed following SDS-PAGE to categorize one dominant protein of 104.7 kDa from the CWPs of JB-1 and equated it with Bacillus spp. endoglucanase with a Mascot score >69. These results point to the potential of using cellular components of probiotics for protection of fish against bacterial diseases.en_UK
dc.publisherBlackwell Publishing-
dc.relationAbbass AA, Sharifuzzaman SM & Austin B (2010) Cellular components of probiotics control Yersinia ruckeri infection in rainbow trout, Oncorhynchus mykiss (Walbaum), Journal of Fish Diseases, 33 (1), pp. 31-37.-
dc.rightsThe publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.-
dc.subjectRainbow trouten_UK
dc.subjectSubcellular componentsen_UK
dc.subjectYersinia ruckerien_UK
dc.titleCellular components of probiotics control Yersinia ruckeri infection in rainbow trout, Oncorhynchus mykiss (Walbaum)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargoreasonThe publisher does not allow this work to be made publicly available in this Repository therefore there is an embargo on the full text of the work.-
dc.citation.jtitleJournal of Fish Diseases-
dc.type.statusPublisher version (final published refereed version)-
dc.contributor.affiliationBenha University-
dc.contributor.affiliationHeriot-Watt University-
Appears in Collections:Aquaculture Journal Articles

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