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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Mitochondrial DNA clocks and the phylogeny of Danaus butterflies
Author(s): Lushai, Gugs
Smith, David A S
Goulson, Dave
Allen, John A
Maclean, Norman
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Keywords: COI
molecular clocks
molecular phylogenetics
mitochondrial DNA
12S rRNA
Issue Date: Dec-2003
Date Deposited: 24-May-2012
Citation: Lushai G, Smith DAS, Goulson D, Allen JA & Maclean N (2003) Mitochondrial DNA clocks and the phylogeny of Danaus butterflies. Insect Science and Its Application, 23 (4), pp. 309-316.
Abstract: Molecular clocks based on sequence change in mitochondrial (mt) DNA have been useful for placing molecular phytogenies in their historical context, thereby enhancing evolutionary insight. Nonetheless, despite their importance to phylogeographers, the methodology is controversial. Here we report on two mitochondrial clocks for the butterfly genus Danaus based on sequences from the cytochrome c oxidase subunit I (COI) and small subunit 12S rRNA (12S) genes. Both clocks are, within the context of Danaus, reliable time-keepers, mutually consistent and, respectively, in agreement with a crustacean COI clock and a molluscan 12S clock. Though we have no fossils with which directly to calibrate sequence divergence rates for Danaus, the 12S molluscan and COI crustacean clocks chosen for comparison were calibrated to radiometrically dated geomorphological events. Our results indicate that the Danaus COI clock evolves approximately four times faster than the 12S clock. Differences between rates of sequence change in terminal sister-taxa are small and likelihood ratio tests do not reject a hypothesis that evolution has been clock-like. The species Danaus chrysippus is paraphyletic and, therefore, invalid. Danaus probably split from its sister-genus Tirumala around 4.9 ± 0.3 million years ago in the early Pliocene.
DOI Link: 10.1017/S1742758400012376
Rights: Published in Insect Science and Its Application. Copyright: Cambridge University Press. Insect Science and Its Application, Volume 23, Issue 4, December 2003, pp. 309 - 316, published by Cambridge University Press. Copyright © Cambridge University Press 2003.

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