Oleispira lenta sp. nov., a novel marine bacterium isolated from Yellow sea coastal seawater in Qingdao, China

Abstract

The taxonomic position of strain DFH11T, which was isolated from coastal seawater off Qingdao, P.R. China in 2007, was determined. Strain DFH11T comprised Gram-negative motile, strictly aerobic spirilli that did not produce catalase. Comparative 16S rRNA gene sequence analysis revealed that strain DFH11T shared ~97% sequence similarity homology with Oleispira antarctica. The strain grew optimally in 2-3% (w/v) NaCl, at pH 5.0-10.0 (optimally at pH 7.0) and between 0 and 30ºC (optimum growth temperature = 28ºC). The major fatty acid was C16:0 (19.2%) and C16:1ω7c and/or iso-C15:0 2-OH (50.4%), and the G + C content of the genomic DNA was 41.5 mol%. Phylogeny based on 16S rRNA gene sequences together with data from phenotypic and chemotaxonomic characterization revealed that DFH11T should be classified as a novel species of the genus Oleispira, for which the name Oleispira lenta sp. nov. is proposed, with the type strain DFH11T (= CGMCC 1.8492T = NCIMB 14529T= LMG 24829T).