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dc.contributor.authorNowell, Reuben Wen_UK
dc.contributor.authorElsworth, Benen_UK
dc.contributor.authorOostra, Vicencioen_UK
dc.contributor.authorZwaan, Bas Jen_UK
dc.contributor.authorWheat, Christopher Wen_UK
dc.contributor.authorSaastamoinen, Marjoen_UK
dc.contributor.authorSaccheri, Ilik Jen_UK
dc.contributor.authorvan’t Hof, Arjen Een_UK
dc.contributor.authorWasik, Bethany Ren_UK
dc.contributor.authorConnahs, Heidien_UK
dc.contributor.authorAslam, Muhammad Len_UK
dc.contributor.authorKumar, Sujaien_UK
dc.contributor.authorChallis, Richard Jen_UK
dc.contributor.authorMonteiro, Antóniaen_UK
dc.contributor.authorBrakefield, Paul Men_UK
dc.contributor.authorBlaxter, Marken_UK
dc.description.abstractThe mycalesine butterfly Bicyclus anynana, the “Squinting bush brown,” is a model organism in the study of lepidopteran ecology, development, and evolution. Here, we present a draft genome sequence for B. anynana to serve as a genomics resource for current and future studies of this important model species. Seven libraries with insert sizes ranging from 350 bp to 20 kb were constructed using DNA from an inbred female and sequenced using both Illumina and PacBio technology; 128 Gb of raw Illumina data was filtered to 124 Gb and assembled to a final size of 475 Mb (∼×260 assembly coverage). Contigs were scaffolded using mate-pair, transcriptome, and PacBio data into 10 800 sequences with an N50 of 638 kb (longest scaffold 5 Mb). The genome is comprised of 26% repetitive elements and encodes a total of 22 642 predicted protein-coding genes. Recovery of a BUSCO set of core metazoan genes was almost complete (98%). Overall, these metrics compare well with other recently published lepidopteran genomes. We report a high-quality draft genome sequence for Bicyclus anynana. The genome assembly and annotated gene models are available at LepBase (
dc.publisherOxford University Press (OUP)en_UK
dc.relationNowell RW, Elsworth B, Oostra V, Zwaan BJ, Wheat CW, Saastamoinen M, Saccheri IJ, van’t Hof AE, Wasik BR, Connahs H, Aslam ML, Kumar S, Challis RJ, Monteiro A, Brakefield PM & Blaxter M (2017) A high-coverage draft genome of the mycalesine butterfly Bicyclus anynana. <i>GigaScience</i>, 6 (7), Art. No.: gix035.
dc.rights© The Authors 2017. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (, which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.subjectComputer Science Applicationsen_UK
dc.subjectHealth Informaticsen_UK
dc.titleA high-coverage draft genome of the mycalesine butterfly Bicyclus anynanaen_UK
dc.typeJournal Articleen_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity College Londonen_UK
dc.contributor.affiliationWageningen Universityen_UK
dc.contributor.affiliationStockholm Universityen_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationUniversity of Liverpoolen_UK
dc.contributor.affiliationUniversity of Liverpoolen_UK
dc.contributor.affiliationYale Universityen_UK
dc.contributor.affiliationNational University of Singaporeen_UK
dc.contributor.affiliationNational University of Singaporeen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
dc.contributor.affiliationYale Universityen_UK
dc.contributor.affiliationUniversity of Cambridgeen_UK
dc.contributor.affiliationUniversity of Edinburghen_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
local.rioxx.authorNowell, Reuben W|0000-0001-7546-6495en_UK
local.rioxx.authorElsworth, Ben|en_UK
local.rioxx.authorOostra, Vicencio|en_UK
local.rioxx.authorZwaan, Bas J|en_UK
local.rioxx.authorWheat, Christopher W|en_UK
local.rioxx.authorSaastamoinen, Marjo|en_UK
local.rioxx.authorSaccheri, Ilik J|en_UK
local.rioxx.authorvan’t Hof, Arjen E|en_UK
local.rioxx.authorWasik, Bethany R|en_UK
local.rioxx.authorConnahs, Heidi|en_UK
local.rioxx.authorAslam, Muhammad L|en_UK
local.rioxx.authorKumar, Sujai|en_UK
local.rioxx.authorChallis, Richard J|en_UK
local.rioxx.authorMonteiro, Antónia|en_UK
local.rioxx.authorBrakefield, Paul M|en_UK
local.rioxx.authorBlaxter, Mark|en_UK
local.rioxx.projectProject ID unknown|Biotechnology and Biological Sciences Research Council|
local.rioxx.filenameNowell 2017 Gigascience - A high-coverage draft genome of mycalesine butterfly Bicyclus anynana.pdfen_UK
Appears in Collections:Biological and Environmental Sciences Journal Articles

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