Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3570
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dc.contributor.authorGregory, Melissa Ken_UK
dc.contributor.authorKing, Hamish Wen_UK
dc.contributor.authorBain, Peter Aen_UK
dc.contributor.authorGibson, Robert Aen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorSchuller, Kathryn Aen_UK
dc.date.accessioned2013-06-09T09:49:08Z-
dc.date.available2013-06-09T09:49:08Z-
dc.date.issued2011-08en_UK
dc.identifier.urihttp://hdl.handle.net/1893/3570-
dc.description.abstractFish oils are rich in omega-3 long-chain polyunsaturated fatty acids (n-3 LC-PUFA), predominantly 20:5n-3 and 22:6n-3, whereas vegetable oils contain abundant C18-PUFA, predominantly 18:3n-3 or 18:2n-6. We hypothesized that replacement of fish oils with vegetable oils would increase the oxidative stability of fish lipids. Here we have used the FHM cell line to test this hypothesis. The FHM cells were readily able to synthesize 20:5n-3 and 24:6n-3 from 18:3n-3 but 22:6n-3 synthesis was negligible. Also, they were readily able to synthesize 20:3n-6 from 18:2n-6 but 20:4n-6 synthesis was negligible. Mitochondrial β-oxidation was greatest for 18:3n-3 and 20:5n-3 and the rates for 16:0, 18:2n-6, 22:6n-3 and 18:1n-9 were significantly lower. Fatty acid incorporation was predominantly into phospholipids (79-97%) with very little incorporation into neutral lipids. Increasing the fatty acid concentration in the growth medium substantially increased the concentrations of 18:3n-3 and 18:2n-6 in the cell phospholipids but this was not the case for 20:5n-3 or 22:6n-3. When they were subjected to oxidative stress, the FHM cells supplemented with either 20:5n-3 or 22:6n-3 (as compared with 18:3n-3 or saturated fatty acids) exhibited significantly higher levels of thiobarbituric reactive substances (TBARS) indicating higher levels of lipid peroxidation. The results are discussed in relation to the effects of fatty acid unsaturation on the oxidative stability of cellular lipids and the implications for sustainable aquaculture.en_UK
dc.language.isoenen_UK
dc.publisherSpringer Verlagen_UK
dc.relationGregory MK, King HW, Bain PA, Gibson RA, Tocher DR & Schuller KA (2011) Development of a fish cell culture model to investigate the impact of fish oil replacement on lipid peroxidation. Lipids, 46 (8), pp. 753-764. https://doi.org/10.1007/s11745-011-3558-9en_UK
dc.rightsPublished in Lipids by Springer Verlag. http://www.springerlink.com/content/ft8q288332441018/. The original publication is available at www.springerlink.comen_UK
dc.subjectAquacultureen_UK
dc.subjectbeta-oxidationen_UK
dc.subjectcell cultureen_UK
dc.subjectfish oilen_UK
dc.subjectlipid peroxidationen_UK
dc.subjectphospholipidsen_UK
dc.subjectpolyunsaturated fatty acidsen_UK
dc.subjectbiosynthesisen_UK
dc.subjectFishes Feeding and feedsen_UK
dc.subjectSustainable fisheriesen_UK
dc.subjectPhospholipidsen_UK
dc.titleDevelopment of a fish cell culture model to investigate the impact of fish oil replacement on lipid peroxidationen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1007/s11745-011-3558-9en_UK
dc.citation.jtitleLipidsen_UK
dc.citation.issn1558-9307en_UK
dc.citation.issn0024-4201en_UK
dc.citation.volume46en_UK
dc.citation.issue8en_UK
dc.citation.spage753en_UK
dc.citation.epage764en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.author.emaildrt1@stir.ac.uken_UK
dc.contributor.affiliationFlinders Universityen_UK
dc.contributor.affiliationFlinders Universityen_UK
dc.contributor.affiliationFlinders Universityen_UK
dc.contributor.affiliationUniversity of Adelaideen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationFlinders Universityen_UK
dc.identifier.isiWOS:000292568000008en_UK
dc.identifier.scopusid2-s2.0-79961209395en_UK
dc.identifier.wtid838016en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.date.accepted1990-01-01en_UK
dcterms.dateAccepted1990-01-01en_UK
dc.date.filedepositdate2012-01-11en_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorGregory, Melissa K|en_UK
local.rioxx.authorKing, Hamish W|en_UK
local.rioxx.authorBain, Peter A|en_UK
local.rioxx.authorGibson, Robert A|en_UK
local.rioxx.authorTocher, Douglas R|0000-0002-8603-9410en_UK
local.rioxx.authorSchuller, Kathryn A|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2012-01-11en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/all-rights-reserved|2012-01-11|en_UK
local.rioxx.filenameGregory et al2011 Final submitted.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0024-4201en_UK
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