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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3569

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Diet x genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon (Salmo salar) in response to replacement of dietary fish oil with vegetable oil
Author(s): Morais, Sofia
Pratoomyot, Jarunan
Torstensen, Bente E
Taggart, John
Guy, Derrick R
Bell, J Gordon
Tocher, Douglas R
Contact Email: drt1@stir.ac.uk
Keywords: Salmon feeds
Aquaculture
Sustainability
Vegetable oil
Fish oil
Alternative feeds
Families
Genetic selection
Liver transcriptome
Gene expression
Issue Date: Nov-2011
Publisher: Cambridge University Press
Citation: Morais S, Pratoomyot J, Torstensen BE, Taggart J, Guy DR, Bell JG & Tocher DR (2011) Diet x genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon (Salmo salar) in response to replacement of dietary fish oil with vegetable oil, British Journal of Nutrition, 106 (10), pp. 1457-1469.
Abstract: The present study investigates the effects of genotype on responses to alternative feeds in Atlantic salmon. Microarray analysis of the liver transcriptome of two family groups, lean or fat, fed a diet containing either a fish oil (FO) or a vegetable oil (VO) blend indicated that pathways of cholesterol and lipoprotein metabolism might be differentially affected by the diet depending on the genetic background of the fish, and this was further investigated by real-time quantitative PCR, plasma and lipoprotein biochemical analysis. Results indicate a reduction in VLDL and LDL levels, with no changes in HDL, when FO is replaced by VO in the lean family group, whereas in fat fish fed FO, levels of apoB-containing lipoproteins were low and comparable with those fed VO in both family groups. Significantly lower levels of plasma TAG and LDL-TAG were measured in the fat group that was independent of diet, whereas plasma cholesterol was significantly higher in fish fed the FO diet in both groups. Hepatic expression of genes involved in cholesterol homeostasis, β-oxidation and lipoprotein metabolism showed relatively subtle changes. A significantly lower expression of genes considered anti-atherogenic in mammals (ATP-binding cassette transporter A1, apoAI, scavenger receptor class B type 1, lipoprotein lipase (LPL)b (TC67836) and LPLc (TC84899)) was found in lean fish, compared with fat fish, when fed VO. Furthermore, the lean family group appeared to show a greater response to diet composition in the cholesterol biosynthesis pathway, mediated by sterol-responsive element-binding protein 2. Finally, the presence of three different transcripts for LPL, with differential patterns of nutritional regulation, was demonstrated.
Type: Journal Article
URI: http://hdl.handle.net/1893/3569
DOI Link: http://dx.doi.org/10.1017/S0007114511001954
Rights: Sofia Morais, Jarunan Pratoomyot, Bente E. Torstensen, John B. Taggart, Derrick R. Guy, J. Gordon Bell and Douglas R. Tocher (2011). Diet × genotype interactions in hepatic cholesterol and lipoprotein metabolism in Atlantic salmon (Salmo salar) in response to replacement of dietary fish oil with vegetable oil. British Journal of Nutrition, 106, pp 1457-1469 doi:10.1017/S0007114511001954.; Published in British Journal of Nutrition. Copyright: Cambridge University Press.; This paper has been accepted for publication and will appear in a revised form, subsequent to editorial input by Cambridge University Press, in British Journal of Nutrition, Volume 106, Issue 10, (November 2011), pp. 1457 - 1469, published by Cambridge University Press, Copyright © Cambridge University Press 2011. http://journals.cambridge.org/action/displayJournal?jid=BJN.
Affiliation: Aquaculture
University of Stirling
National Institute of Nutrition and Seafood Research (NIFES)
Aquaculture
Landcatch Natural Selection Ltd
Aquaculture
Aquaculture

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