|Appears in Collections:
|Biological and Environmental Sciences Journal Articles
|Peer Review Status:
|What mandrills leave behind: using fecal samples to characterize the major histocompatibility complex in a threatened primate
van Oosterhout, Cock
Guibinga Mickala, Amour
Ecology, Evolution, Behavior and Systematics
|Weber A, Lighten J, van Oosterhout C, Guibinga Mickala A, Ntie S, Mickala P, Lehmann D, Abernethy K & Anthony N (2023) What mandrills leave behind: using fecal samples to characterize the major histocompatibility complex in a threatened primate. <i>Conservation Genetics</i>. https://doi.org/10.1007/s10592-023-01587-2
|The major histocompatibility complex (MHC) can be useful in guiding conservation planning because of its influence on immunity, fitness, and reproductive ecology in vertebrates. The mandrill (Mandrillus sphinx) is a threatened primate endemic to central Africa. Considerable research in this species has shown that the MHC is important for disease resistance, mate choice, and reproductive success. However, all previous MHC research in mandrills has focused on an inbred semi-captive population, so their genetic diversity may have been underestimated. Here we expand our current knowledge of mandrill MHC variation by performing next-generation sequencing of non-invasively collected fecal samples from a large wild horde in central Gabon. We observe MHC lineages and alleles shared with other primates, and we uncover 45 putative new class II MHC DRB alleles, including representatives of the DRB9 pseudogene, which has not previously been identified in mandrills. We also document methodological challenges associated with fecal samples in NGS-based MHC research. Even with high read depth, the replicability of alleles from fecal samples was lower than that of tissue samples, and allele assignments are inconsistent between sample types. Further, the common assumption that variants with very high read depth should represent true alleles does not appear to be reliable for fecal samples. Nevertheless, the use of degraded DNA in the present study still enabled significant progress in quantifying immunogenetic diversity and its evolution in wild primates.
|This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is a post-peer-review, pre-copyedit version of an article published in Conservation Genetics. The final authenticated version is available online at: https://doi.org/10.1007/s10592-023-01587-2
|Output Status: Forthcoming/Available Online
|Fulltext - Accepted Version
|Under Embargo until 2024-11-29 Request a copy
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.