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Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: The molecular basis for pore pattern morphogenesis in diatom silica
Author(s): Heintze, Christoph
Babenko, Iaroslav
Zackova Suchanova, Jirina
Skeffington, Alastair
Friedrich, Benjamin M.
Kröger, Nils
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Keywords: Biomineralization
Mesoporous Silica
Silica Deposition Vesicle
Phase Separation
Ankyrin-Repeat Domain
Issue Date: 6-Dec-2022
Date Deposited: 27-Jun-2023
Citation: Heintze C, Babenko I, Zackova Suchanova J, Skeffington A, Friedrich BM & Kröger N (2022) The molecular basis for pore pattern morphogenesis in diatom silica. <i>Proceedings of the National Academy of Sciences</i>, 119 (49).
Abstract: Biomineral-forming organisms produce inorganic materials with complex, genetically encoded morphologies that are unmatched by current synthetic chemistry. It is poorly understood which genes are involved in biomineral morphogenesis and how the encoded proteins guide this process. We addressed these questions using diatoms, which are paradigms for the self-assembly of hierarchically meso- and macroporous silica under mild reaction conditions. Proteomics analysis of the intracellular organelle for silica biosynthesis led to the identification of new biomineralization proteins. Three of these, coined dAnk1-3, contain a common protein–protein interaction domain (ankyrin repeats), indicating a role in coordinating assembly of the silica biomineralization machinery. Knocking out individual dank genes led to aberrations in silica biogenesis that are consistent with liquid–liquid phase separation as underlying mechanism for pore pattern morphogenesis. Our work provides an unprecedented path for the synthesis of tailored mesoporous silica materials using synthetic biology.
DOI Link: 10.1073/pnas.2211549119
Rights: Copyright © 2022 the Author(s). Published by PNAS. This article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).
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