Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/34782
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dc.contributor.authorElbialy, Zizy Ien_UK
dc.contributor.authorAtiba, Aymanen_UK
dc.contributor.authorAbdelnaby, Amlen_UK
dc.contributor.authorAl-Hawary, Ibrahim Ien_UK
dc.contributor.authorElsheshtawy, Ahmeden_UK
dc.contributor.authorEl-Serehy, Hamed Aen_UK
dc.contributor.authorAbdel-Daim, Mohamed Men_UK
dc.contributor.authorFadl, Sabreen Een_UK
dc.contributor.authorAssar, Doaa Hen_UK
dc.date.accessioned2023-02-03T01:10:17Z-
dc.date.available2023-02-03T01:10:17Z-
dc.date.issued2020-12en_UK
dc.identifier.other352en_UK
dc.identifier.urihttp://hdl.handle.net/1893/34782-
dc.description.abstractBackground Collagen is the most abundant structural protein in the mammalian connective tissue and represents approximately 30% of animal protein. The current study evaluated the potential capacity of collagen extract derived from Nile tilapia skin in improving the cutaneous wound healing in rats and investigated the underlying possible mechanisms. A rat model was used, and the experimental design included a control group (CG) and the tilapia collagen treated group (TCG). Full-thickness wounds were conducted on the back of all the rats under general anesthesia, then the tilapia collagen extract was applied topically on the wound area of TCG. Wound areas of the two experimental groups were measured on days 0, 3, 6, 9, 12, and 15 post-wounding. The stages of the wound granulation tissues were detected by histopathologic examination and the expression of vascular endothelial growth factor (VEGF), and transforming growth factor (TGF-ß1) were investigated using immunohistochemistry. Moreover, relative gene expression analysis of transforming growth factor-beta (TGF-ß1), basic fibroblast growth factor (bFGF), and alpha-smooth muscle actin (α-SMA) were quantified by real-time qPCR. Results The histopathological assessment showed noticeable signs of skin healing in TCG compared to CG. Immunohistochemistry results revealed remarkable enhancement in the expression levels of VEGF and TGF-β1 in TCG. Furthermore, TCG exhibited marked upregulation in the VEGF, bFGF, and α-SMA genes expression. These findings suggested that the topical application of Nile tilapia collagen extract can promote the cutaneous wound healing process in rats, which could be attributed to its stimulating effect on recruiting and activating macrophages to produce chemotactic growth factors, fibroblast proliferation, and angiogenesis. Conclusions The collagen extract could, therefore, be a potential biomaterial for cutaneous wound healing therapeutics. Backgrounden_UK
dc.language.isoenen_UK
dc.publisherSpringer Science and Business Media LLCen_UK
dc.relationElbialy ZI, Atiba A, Abdelnaby A, Al-Hawary II, Elsheshtawy A, El-Serehy HA, Abdel-Daim MM, Fadl SE & Assar DH (2020) Collagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expression. <i>BMC Veterinary Research</i>, 16 (1), Art. No.: 352. https://doi.org/10.1186/s12917-020-02566-2en_UK
dc.rightsOpen Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectTilapia collagenen_UK
dc.subjectWound healingen_UK
dc.subjectVEGFen_UK
dc.subjectbFGFen_UK
dc.subjectTGF-ßen_UK
dc.subjectα-SMAen_UK
dc.subjectIHCen_UK
dc.subjectHistopathologyen_UK
dc.subjectGene expressionen_UK
dc.titleCollagen extract obtained from Nile tilapia (Oreochromis niloticus L.) skin accelerates wound healing in rat model via up regulating VEGF, bFGF, and α-SMA genes expressionen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/s12917-020-02566-2en_UK
dc.identifier.pmid32972407en_UK
dc.citation.jtitleBMC Veterinary Researchen_UK
dc.citation.issn1746-6148en_UK
dc.citation.volume16en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderKing Saud Universityen_UK
dc.citation.date24/09/2020en_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.contributor.affiliationKing Saud University, Saudi Arabiaen_UK
dc.contributor.affiliationKing Saud University, Saudi Arabiaen_UK
dc.contributor.affiliationMatrouh Universityen_UK
dc.contributor.affiliationKafrelsheik Universityen_UK
dc.identifier.isiWOS:000576131300001en_UK
dc.identifier.scopusid2-s2.0-85091622042en_UK
dc.identifier.wtid1863059en_UK
dc.contributor.orcid0000-0003-3811-4997en_UK
dc.contributor.orcid0000-0002-4341-2713en_UK
dc.date.accepted2020-09-11en_UK
dcterms.dateAccepted2020-09-11en_UK
dc.date.filedepositdate2023-01-06en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorElbialy, Zizy I|en_UK
local.rioxx.authorAtiba, Ayman|en_UK
local.rioxx.authorAbdelnaby, Aml|en_UK
local.rioxx.authorAl-Hawary, Ibrahim I|en_UK
local.rioxx.authorElsheshtawy, Ahmed|0000-0003-3811-4997en_UK
local.rioxx.authorEl-Serehy, Hamed A|en_UK
local.rioxx.authorAbdel-Daim, Mohamed M|0000-0002-4341-2713en_UK
local.rioxx.authorFadl, Sabreen E|en_UK
local.rioxx.authorAssar, Doaa H|en_UK
local.rioxx.projectRSP-2020/19|King Saud University|en_UK
local.rioxx.freetoreaddate2023-01-06en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2023-01-06|en_UK
local.rioxx.filenames12917-020-02566-2.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1746-6148en_UK
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