Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/34543
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Investigating the impacts of H(2)O(2) treatment on gills of healthy Atlantic salmon reveals potential changes to mucus production with implications on immune activity
Author(s): Fernandez-Senac, Carolina
Monaghan, Sean J
Mascolo, Dario
Baily, Johanna L
Betancor, Monica
Chalmers, Lynn
Paladini, Giuseppe
Adams, Alexandra
Fridman, Sophie
Bron, James E
Contact Email: m.b.betancor@stir.ac.uk
Issue Date: Sep-2022
Date Deposited: 1-Aug-2022
Citation: Fernandez-Senac C, Monaghan SJ, Mascolo D, Baily JL, Betancor M, Chalmers L, Paladini G, Adams A, Fridman S & Bron JE (2022) Investigating the impacts of H(2)O(2) treatment on gills of healthy Atlantic salmon reveals potential changes to mucus production with implications on immune activity. Fish and Shellfish Immunology, 128, pp. 74-81. https://doi.org/10.1016/j.fsi.2022.06.063
Abstract: Current treatment strategies for relevant infectious diseases in Atlantic salmon (Salmo salar L.) include the use of low salinity or freshwater bathing. However, often availability is restricted, and hydrogen peroxide (H2O2) is used as an alternative. The potential impacts of H2O2 on fish mucosal tissues, especially the gills therefore need to be considered. In this study the mucosal and immunological effects of H2O2 treatment on the gills of healthy Atlantic salmon were examined by gene expression (qPCR) and immunohistochemistry (IHC) investigating T-cell, B-cell, and mucin activity. Healthy fish were treated with H2O2 and sampled at different times: 4 h, 24 h and 14 days post-H2O2 treatment (dpt) (total n = 18) to investigate the effect of holding time and H2O2 treatment. Treatment with H2O2 resulted in up-regulation of markers for T-cell activity and anti-inflammatory response and down-regulation of mucin expression in the gills at 14 dpt compared to fish sampled prior to treatment (0h; n = 5 fish). These findings were supported by IHC analysis, which despite being highly variable between samples, showed an increase in the number of CD3+ T cells at 14 dpt in 50% of treated fish compared to pre-treatment fish. The results from this study suggest that H2O2 treatment does not immune compromise healthy Atlantic salmon after 14 dpt (i.e., post-recovery) but modulates gill immune activity and disrupts the mucus covering of the gills. However, further studies are required to determine whether the effects observed are related to H2O2 treatment in isolation or other variables such as holding time or environmental factors.
DOI Link: 10.1016/j.fsi.2022.06.063
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Fernandez-Senac C, Monaghan SJ, Mascolo D, Baily JL, Betancor M, Chalmers L, Paladini G, Adams A, Fridman S & Bron JE (2022) Investigating the impacts of H(2)O(2) treatment on gills of healthy Atlantic salmon reveals potential changes to mucus production with implications on immune activity. Fish and Shellfish Immunology, 128, pp. 74-81. https://doi.org/10.1016/j.fsi.2022.06.063 © 2022, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/
Licence URL(s): http://creativecommons.org/licenses/by-nc-nd/4.0/

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