|Appears in Collections:||Biological and Environmental Sciences Journal Articles|
|Peer Review Status:||Refereed|
|Title:||A new method for using 18O to trace ozone deposition|
Emberson, Lisa D
Barnes, Jeremy D
Evershed, Richard P
Ozone depleting substances.
|Citation:||Subke J, Toet S, D’Haese D, Crossman Z, Emberson LD, Barnes JD, Evershed RP & Ineson P (2009) A new method for using 18O to trace ozone deposition. Rapid Communications in Mass Spectrometry, 23 (7), pp. 980-984. https://doi.org/10.1002/rcm.3961|
|Abstract:||Isotopically labelled ozone (18O3) is an ideal tool to study the deposition of O3 to plants and soil, but no studies have made use of it due to the technical difficulties in producing isotopically enriched ozone. For 18O3 to be used in fumigation experiments, it has to be purified and stored safely prior to fumigations, to ensure that the label is present predominantly in the form ofO3, and to make efficient use of isotopically highly enriched oxygen. We present a simple apparatus that allows for the safe generation, purification, storage, and release of 18O3. Following the purification and release of O3, about half (by volume) of the 18O is present in the form of O3. This means that for a given release of 18O3 into the fumigation system, a roughly identical volume of 18O2 is released. However, the small volume of this concurrent 18O2 release (100 nmol molS1 in our experiment) results in only a minor shift of the much larger atmospheric oxygen pool, with no detectable consequence for the isotopic enrichment of either soil or plant materials. We demonstrate here the feasibility of using 18O as an isotopic tracer inO3 fumigations by exposing dry soil to 100 nmol molS1 18O3 for periods ranging from 1 to 11 h. The 18O tracer accumulation in soil samples is measured using gas chromatography/isotope ratio mass spectrometry (GC/IRMS), and the results show a linear increase in 18O/16O isotope ratio over time, with significant differences detectable after 1 h of exposure. The apparatus is adapted for use with fumigation chambers sustaining flow rates of 1m3 minS1 for up to 12 h, but simple modifications now allow larger quantities of O3 to be stored and continuously released (e.g. for use with open-top chambers or FACE facilities).|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author; you can only request a copy if you wish to use this work for your own research or private study.|
|Subke et al 2009 RCMS.pdf||Fulltext - Published Version||118.16 kB||Adobe PDF||Under Permanent Embargo Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
The metadata of the records in the Repository are available under the CC0 public domain dedication: No Rights Reserved https://creativecommons.org/publicdomain/zero/1.0/
If you believe that any material held in STORRE infringes copyright, please contact firstname.lastname@example.org providing details and we will remove the Work from public display in STORRE and investigate your claim.