Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/32703
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dc.contributor.authorZhang, Xiao-Huaen_UK
dc.contributor.authorAhmad, Waqaren_UK
dc.contributor.authorZhu, Xiao-Yuen_UK
dc.contributor.authorChen, Jiaxingen_UK
dc.contributor.authorAustin, Brianen_UK
dc.date.accessioned2021-06-11T15:34:52Z-
dc.date.available2021-06-11T15:34:52Z-
dc.date.issued2021-05en_UK
dc.identifier.urihttp://hdl.handle.net/1893/32703-
dc.description.abstractCulturing has been the cornerstone of microbiology since Robert Koch first successfully cultured bacteria in the late nineteenth century. However, even today, the majority of microorganisms in the marine environment remain uncultivated. There are various explanations for the inability to culture bacteria in the laboratory, including lack of essential nutrients, osmotic support or incubation conditions, low growth rate, development of micro-colonies, and the presence of senescent or viable but nonculturable (VBNC) cells. In the marine environment, many bacteria have been associated with dormancy, as typified by the VBNC state. VBNC refers to a state where bacteria are metabolically active, but are no longer culturable on routine growth media. It is apparently a unique survival strategy that has been adopted by many microorganisms in response to harsh environmental conditions and the bacterial cells in the VBNC state may regain culturability under favorable conditions. The resuscitation of VBNC cells may well be an important way to cultivate the otherwise uncultured microorganisms in marine environments. Many resuscitation stimuli that promote the restoration of culturability have so far been identified; these include sodium pyruvate, quorum sensing autoinducers, resuscitation-promoting factors Rpfs and YeaZ, and catalase. In this review, we focus on the issues associated with bacterial culturability, the diversity of bacteria entering the VBNC state, mechanisms of induction into the VBNC state, resuscitation factors of VBNC cells and implications of VBNC resuscitation stimuli for cultivating these otherwise uncultured microorganisms. Bringing important microorganisms into culture is still important in the era of high-throughput sequencing as their ecological functions in the marine environment can often only be known through isolation and cultivation.en_UK
dc.language.isoenen_UK
dc.publisherSpringer (part of Springer Nature)en_UK
dc.relationZhang X, Ahmad W, Zhu X, Chen J & Austin B (2021) Viable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganisms. Marine Life Science and Technology, 3 (2), pp. 189-203. https://doi.org/10.1007/s42995-020-00041-3en_UK
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectVBNCen_UK
dc.subjectCultivating the uncultureden_UK
dc.subjectResuscitationen_UK
dc.subjectMarine microorganismsen_UK
dc.titleViable but nonculturable bacteria and their resuscitation: implications for cultivating uncultured marine microorganismsen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1007/s42995-020-00041-3en_UK
dc.citation.jtitleMarine Life Science and Technologyen_UK
dc.citation.issn2662-1746en_UK
dc.citation.volume3en_UK
dc.citation.issue2en_UK
dc.citation.spage189en_UK
dc.citation.epage203en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.citation.date13/05/2020en_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationOcean University of Chinaen_UK
dc.contributor.affiliationLanzhou University of Technologyen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000649455400007en_UK
dc.identifier.wtid1735245en_UK
dc.date.accepted2020-03-25en_UK
dcterms.dateAccepted2020-03-25en_UK
dc.date.filedepositdate2021-06-11en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorZhang, Xiao-Hua|en_UK
local.rioxx.authorAhmad, Waqar|en_UK
local.rioxx.authorZhu, Xiao-Yu|en_UK
local.rioxx.authorChen, Jiaxing|en_UK
local.rioxx.authorAustin, Brian|en_UK
local.rioxx.projectInternal Project|University of Stirling|https://isni.org/isni/0000000122484331en_UK
local.rioxx.freetoreaddate2021-06-11en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2021-06-11|en_UK
local.rioxx.filenameZhang2021_Article_ViableButNonculturableBacteria.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source2662-1746en_UK
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