Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/32251
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dc.contributor.authorKrachler, Anne Marieen_UK
dc.contributor.authorSirisaengtaksin, Natalieen_UK
dc.contributor.authorMonteith, Paulineen_UK
dc.contributor.authorPaine, C E Timothyen_UK
dc.contributor.authorCoates, Christopher Jen_UK
dc.contributor.authorLim, Jensonen_UK
dc.date.accessioned2021-02-09T01:00:47Z-
dc.date.available2021-02-09T01:00:47Z-
dc.date.issued2021en_UK
dc.identifier.urihttp://hdl.handle.net/1893/32251-
dc.description.abstractAdhesins facilitate bacterial colonization and invasion of host tissues and are considered virulence factors, but their impact on immune-mediated damage as a driver of pathogenesis remains unclear. Yersinia pseudotuberculosis encodes for a multivalent adhesion molecule (MAM), a mammalian cell entry (MCE) family protein and adhesin. MAMs are widespread in Gram-negative bacteria and enable enteric bacteria to colonize epithelial tissues. Their role in bacterial interactions with the host innate immune system and contribution to pathogenicity remains unclear. Here, we investigated how Y. pseudotuberculosis MAM contributes to pathogenesis during infection of the Galleria mellonella insect model. We show that Y. pseudotuberculosis MAM is required for efficient bacterial binding and uptake by hemocytes, the host phagocytes. Y. pseudotuberculosis interactions with insect and mammalian phagocytes are determined by bacterial and host factors. Loss of MAM, and deficient microbe–phagocyte interaction, increased pathogenesis in G. mellonella. Diminished phagocyte association also led to increased bacterial clearance. Furthermore, Y. pseudotuberculosis that failed to engage phagocytes hyperactivated humoral immune responses, most notably melanin production. Despite clearing the pathogen, excessive melanization also increased phagocyte death and host mortality. Our findings provide a basis for further studies investigating how microbe- and host-factors integrate to drive pathogenesis in a tractable experimental system.en_UK
dc.language.isoenen_UK
dc.publisherTaylor & Francisen_UK
dc.relationKrachler AM, Sirisaengtaksin N, Monteith P, Paine CET, Coates CJ & Lim J (2021) Defective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbe. Virulence, 12 (1), pp. 638-353. https://doi.org/10.1080/21505594.2021.1878672en_UK
dc.rights© 2021 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectYersiniaen_UK
dc.subjectGalleriaen_UK
dc.subjectadhesinen_UK
dc.subjectinnate immunityen_UK
dc.subjectmelanogenesisen_UK
dc.subjectdamage response frameworken_UK
dc.titleDefective phagocyte association during infection of Galleria mellonella with Yersinia pseudotuberculosis is detrimental to both insect host and microbeen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1080/21505594.2021.1878672en_UK
dc.identifier.pmid33550901en_UK
dc.citation.jtitleVirulenceen_UK
dc.citation.issn2150-5608en_UK
dc.citation.issn2150-5594en_UK
dc.citation.volume12en_UK
dc.citation.issue1en_UK
dc.citation.spage638en_UK
dc.citation.epage353en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderThe Carnegie Trusten_UK
dc.contributor.funderBiotechnology and Biological Sciences Research Councilen_UK
dc.citation.date08/02/2021en_UK
dc.contributor.affiliationUniversity of Texasen_UK
dc.contributor.affiliationUniversity of Texasen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.contributor.affiliationUniversity of New Englanden_UK
dc.contributor.affiliationSwansea Universityen_UK
dc.contributor.affiliationBiological and Environmental Sciencesen_UK
dc.identifier.isiWOS:000616172300001en_UK
dc.identifier.scopusid2-s2.0-85100701851en_UK
dc.identifier.wtid1703586en_UK
dc.contributor.orcid0000-0002-1447-9484en_UK
dc.contributor.orcid0000-0001-7417-356Xen_UK
dc.date.accepted2021-01-15en_UK
dcterms.dateAccepted2021-01-15en_UK
dc.date.filedepositdate2021-02-08en_UK
dc.relation.funderprojectEvaluating the impact of chronic exposure to low dose ionising radiation on immunityen_UK
dc.relation.funderrefRIG008296en_UK
rioxxterms.apcpaiden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorKrachler, Anne Marie|en_UK
local.rioxx.authorSirisaengtaksin, Natalie|en_UK
local.rioxx.authorMonteith, Pauline|0000-0002-1447-9484en_UK
local.rioxx.authorPaine, C E Timothy|en_UK
local.rioxx.authorCoates, Christopher J|en_UK
local.rioxx.authorLim, Jenson|0000-0001-7417-356Xen_UK
local.rioxx.projectRIG008296|The Carnegie Trust|en_UK
local.rioxx.freetoreaddate2021-02-08en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2021-02-08|en_UK
local.rioxx.filenameKrachler-etal-Virulence-2021.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source2150-5608en_UK
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