Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31502
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dc.contributor.authorCoyle, Nicola Men_UK
dc.contributor.authorBartie, Kerry Len_UK
dc.contributor.authorBayliss, Sion Cen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorMcMillan, Stuarten_UK
dc.contributor.authorVerner-Jeffreys, David Wen_UK
dc.contributor.authorDesbois, Andrew Pen_UK
dc.contributor.authorFeil, Edward Jen_UK
dc.date.accessioned2020-08-01T00:03:18Z-
dc.date.available2020-08-01T00:03:18Z-
dc.date.issued2020en_UK
dc.identifier.other1430en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31502-
dc.description.abstractVibrio anguillarum is the causative agent of vibriosis in many species important to aquaculture. We generated whole genome sequence (WGS) data on a diverse collection of 64 V. anguillarum strains, which we supplemented with 41 publicly available genomes to produce a combined dataset of 105 strains. These WGS data resolved six major lineages (L1-L6), and the additional use of multilocus sequence analysis (MLSA) clarified the association of L1 with serotype O1 and Salmonidae hosts (salmon/trout), and L2 with serotypes O2a/O2b/O2c and Gadidae hosts (cod). Our analysis also revealed a large-scale homologous replacement of 526-kb of core genome in an L2 strain from a con-specific donor. Although the strains affected by this recombination event are exclusively associated with Gadidae, we find no clear genetic evidence that it has played a causal role in host specialism. Whilst it is established that Vibrio species freely recombine, to our knowledge this is the first report of a contiguous recombinational replacement of this magnitude in any Vibrio genome. We also note a smaller accessory region of high single nucleotide polymorphism (SNP) density and gene content variation that contains lipopolysaccharide biosynthesis genes which may play a role in determining serotype.en_UK
dc.language.isoenen_UK
dc.publisherFrontiers Mediaen_UK
dc.relationCoyle NM, Bartie KL, Bayliss SC, Bekaert M, Adams A, McMillan S, Verner-Jeffreys DW, Desbois AP & Feil EJ (2020) A Hopeful Sea-Monster: A Very Large Homologous Recombination Event Impacting the Core Genome of the Marine Pathogen Vibrio anguillarum. Frontiers in Microbiology, 11, Art. No.: 1430. https://doi.org/10.3389/fmicb.2020.01430en_UK
dc.rights© 2020 Coyle, Bartie, Bayliss, Bekaert, Adams, McMillan, Verner-Jeffreys, Desbois and Feil. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY - https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectVibrio anguillarumen_UK
dc.subjectwhole genome sequencingen_UK
dc.subjectpopulation structureen_UK
dc.subjectrecombinationen_UK
dc.subjectadaptationen_UK
dc.titleA Hopeful Sea-Monster: A Very Large Homologous Recombination Event Impacting the Core Genome of the Marine Pathogen Vibrio anguillarumen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.3389/fmicb.2020.01430en_UK
dc.identifier.pmid32695083en_UK
dc.citation.jtitleFrontiers in Microbiologyen_UK
dc.citation.issn1664-302Xen_UK
dc.citation.volume11en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBBSRC Biotechnology and Biological Sciences Research Councilen_UK
dc.citation.date29/06/2020en_UK
dc.contributor.affiliationUniversity of Bathen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Bathen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCEFAS - Centre for Environment, Fisheries and Aquaculture Scienceen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Bathen_UK
dc.identifier.scopusid2-s2.0-85087830751en_UK
dc.identifier.wtid1648780en_UK
dc.contributor.orcid0000-0003-3037-5295en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0003-2368-7864en_UK
dc.contributor.orcid0000-0001-6052-8761en_UK
dc.date.accepted2020-06-02en_UK
dc.date.filedepositdate2020-07-31en_UK
dc.relation.funderprojectCapacity building for the widespread adoption of whole genome sequencing (WGS) for the molecularen_UK
dc.relation.funderrefBB/M026388/1en_UK
Appears in Collections:Aquaculture Journal Articles

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