Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31474
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dc.contributor.authorBartie, Kerry Len_UK
dc.contributor.authorTaslima, Khanamen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorWehner, Stefanieen_UK
dc.contributor.authorSyaifudin, Mochamaden_UK
dc.contributor.authorTaggart, John Ben_UK
dc.contributor.authorde Verdal, Huguesen_UK
dc.contributor.authorRosario, Westlyen_UK
dc.contributor.authorMuyalde, Nerafeen_UK
dc.contributor.authorBenzie, John A Hen_UK
dc.contributor.authorMcAndrew, Brendan Jen_UK
dc.contributor.authorPenman, David Jen_UK
dc.date.accessioned2020-07-22T00:08:47Z-
dc.date.available2020-07-22T00:08:47Z-
dc.date.issued2020-09-15en_UK
dc.identifier.other735433en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31474-
dc.description.abstractThe “Molobicus” hybrid breeding programme was initiated to improve tilapia growth performance in brackish water. The base population was created by backcrossing F1 Oreochromis niloticus GIFT strains × feral O. mossambicus to O. mossambicus and selective breeding conducted for performance in brackish water with two lines selected in extensive or intensive rearing conditions. A panel of ten diagnostic SNP markers was applied to estimate the species composition at different stages in the Molobicus programme including parental stocks, F1 and seventh generation fish from the selective lines. The O. aureus-specific markers tested revealed zero or negligible contribution from O. aureus to all the groups analysed. Feral O. mossambicus possessed an estimated 0.98 frequency of O. mossambicus-specific alleles, while GIFT samples had an estimated mean frequency of 0.88 O. niloticus-specific alleles. Hybrid F1 GIFT × feral O. mossambicus samples demonstrated close to 50:50 allele frequencies from O. niloticus and O. mossambicus for seven of the eight SNP loci tested. Analysis of the combined seventh generation Molobicus samples revealed a significant excess of O. niloticus alleles in six out of the eight SNPs tested, with this trend being more pronounced in the line selected in intensive culture conditions and showing increased body weight. PCR-based SNP assays such as these can be used to inform on the individual species contribution of fish stocks and provide tools for the genetic management of the tilapia species and future breeding programmes.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationBartie KL, Taslima K, Bekaert M, Wehner S, Syaifudin M, Taggart JB, de Verdal H, Rosario W, Muyalde N, Benzie JAH, McAndrew BJ & Penman DJ (2020) Species composition in the Molobicus hybrid tilapia strain. Aquaculture, 526, Art. No.: 735433. https://doi.org/10.1016/j.aquaculture.2020.735433en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Bartie KL, Taslima K, Bekaert M, Wehner S, Syaifudin M, Taggart JB, de Verdal H, Rosario W, Muyalde N, Benzie JAH, McAndrew BJ & Penman DJ (2020) Species composition in the Molobicus hybrid tilapia strain. Aquaculture, 526, Art. No.: 735433. https://doi.org/10.1016/j.aquaculture.2020.735433 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectTilapiaen_UK
dc.subjectGIFTen_UK
dc.subjectMolobicusen_UK
dc.subjectSelective breedingen_UK
dc.subjectSpecies-specific SNP markersen_UK
dc.subjectAquacultureen_UK
dc.titleSpecies composition in the Molobicus hybrid tilapia strainen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2021-05-05en_UK
dc.rights.embargoreason[Molobicus_Aquaculture.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2020.735433en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume526en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderMarine Alliance for Science & Technology Scotlanden_UK
dc.author.emailmichael.bekaert@stir.ac.uken_UK
dc.citation.date04/05/2020en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationBangladesh Agricultural Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationWorldFishen_UK
dc.contributor.affiliationBureau of Fisheries and Aquatic Resources (BFAR-NIFTDC)en_UK
dc.contributor.affiliationBureau of Fisheries and Aquatic Resources (BFAR-NIFTDC)en_UK
dc.contributor.affiliationCIRADen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000536868500011en_UK
dc.identifier.scopusid2-s2.0-85084249673en_UK
dc.identifier.wtid1622209en_UK
dc.contributor.orcid0000-0003-3037-5295en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-3632-2584en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dc.contributor.orcid0000-0001-7384-5133en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dc.date.accepted2020-04-30en_UK
dcterms.dateAccepted2020-04-30en_UK
dc.date.filedepositdate2020-07-20en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorBartie, Kerry L|0000-0003-3037-5295en_UK
local.rioxx.authorTaslima, Khanam|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorWehner, Stefanie|0000-0002-3632-2584en_UK
local.rioxx.authorSyaifudin, Mochamad|en_UK
local.rioxx.authorTaggart, John B|0000-0002-3843-9663en_UK
local.rioxx.authorde Verdal, Hugues|en_UK
local.rioxx.authorRosario, Westly|en_UK
local.rioxx.authorMuyalde, Nerafe|en_UK
local.rioxx.authorBenzie, John A H|en_UK
local.rioxx.authorMcAndrew, Brendan J|0000-0001-7384-5133en_UK
local.rioxx.authorPenman, David J|0000-0001-8608-6631en_UK
local.rioxx.projectProject ID unknown|Marine Alliance for Science & Technology Scotland|en_UK
local.rioxx.freetoreaddate2021-05-05en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2021-05-04en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2021-05-05|en_UK
local.rioxx.filenameMolobicus_Aquaculture.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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