Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31450
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dc.contributor.authorJääskeläinen, Anne Jen_UK
dc.contributor.authorSironen, Tarjaen_UK
dc.contributor.authorKaloinen, Minttuen_UK
dc.contributor.authorKakkola, Lauraen_UK
dc.contributor.authorJulkunen, Ilkkaen_UK
dc.contributor.authorHewson, Rogeren_UK
dc.contributor.authorWeidmann, Manfreden_UK
dc.contributor.authorMirazimi, Alien_UK
dc.contributor.authorWatson, Roberten_UK
dc.contributor.authorVapalahti, Ollien_UK
dc.date.accessioned2020-07-18T00:12:11Z-
dc.date.available2020-07-18T00:12:11Z-
dc.date.issued2020-10en_UK
dc.identifier.other113941en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31450-
dc.description.abstractIn last five years, the Africa has faced two outbreaks of Zaire ebolavirus. These outbreaks have been the largest so far, and latest outbreak is still ongoing and affecting the Democratic Republic of the Congo. We tested in parallel three different Zaire ebolavirus (EBOV) realtime RT-PCRs targeting the nucleoprotein gene (EBOV NP-RT-qPCRs) described by Trombley et al. (2010), Huang et al. (2012) and Weidmann et al. (2004). These assays are used regularly in diagnostic laboratories. The limit of detection (LOD), intra-assay repeatability using different matrixes, sensitivity and specificity were determined. In addition, the primers and probes were aligned with the sequences available in ongoing and past outbreaks in order to check the mismatches. The specificity of all three EBOV NP-RT-qPCRs were excellent (100%), and LODs were under or 10 copies per PCR reaction. Intra-assay repeatability was good in all assays, however the Ct-values were bit higher using the EDTA-blood based matrix. All of the primers and probes in EBOV NP-RTqPCR assays have one or more mismatches in the probes and primers when the 2267 Zaire EBOV NP sequences, including strains Ituri from DRC outbreak (year 2018), was aligned. The EBOV strain of Bikoro (year 2018) circulating in DRC was 100% match in Trombley and Weidmann assay, but had one mismatch in Huang assay.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationJääskeläinen AJ, Sironen T, Kaloinen M, Kakkola L, Julkunen I, Hewson R, Weidmann M, Mirazimi A, Watson R & Vapalahti O (2020) Comparison of Zaire ebolavirus realtime RT-PCRs targeting the nucleoprotein gene. Journal of Virological Methods, 284, Art. No.: 113941. https://doi.org/10.1016/j.jviromet.2020.113941en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Jääskeläinen AJ, Sironen T, Kaloinen M, Kakkola L, Julkunen I, Hewson R, Weidmann M, Mirazimi A, Watson R & Vapalahti O (2020) Comparison of Zaire ebolavirus realtime RT-PCRs targeting the nucleoprotein gene. Journal of Virological Methods, 284, Art. No.: 113941. https://doi.org/10.1016/j.jviromet.2020.113941 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectEbolaen_UK
dc.subjectZaireen_UK
dc.subjectPCRen_UK
dc.subjectnucleoproteinen_UK
dc.titleComparison of Zaire ebolavirus realtime RT-PCRs targeting the nucleoprotein geneen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2022-01-23en_UK
dc.rights.embargoreason[Jaaskelainen AJ et al.pdf] Publisher requires embargo of 18 months after formal publication.en_UK
dc.identifier.doi10.1016/j.jviromet.2020.113941en_UK
dc.identifier.pmid32707049en_UK
dc.citation.jtitleJournal of Virological Methodsen_UK
dc.citation.issn1879-0984en_UK
dc.citation.issn0166-0934en_UK
dc.citation.volume284en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderEuropean Commission (Horizon 2020)en_UK
dc.author.emailm.w.weidmann@stir.ac.uken_UK
dc.citation.date22/07/2020en_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.contributor.affiliationUniversity of Turkuen_UK
dc.contributor.affiliationUniversity of Turkuen_UK
dc.contributor.affiliationPublic Health Englanden_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationPublic Health Agency of Swedenen_UK
dc.contributor.affiliationPublic Health Englanden_UK
dc.contributor.affiliationUniversity of Helsinkien_UK
dc.identifier.isiWOS:000564505200013en_UK
dc.identifier.scopusid2-s2.0-85088498446en_UK
dc.identifier.wtid1645789en_UK
dc.contributor.orcid0000-0002-7063-7491en_UK
dc.date.accepted2020-07-17en_UK
dc.date.filedepositdate2020-07-17en_UK
dc.relation.funderprojectEbola Virus: Modern approaches for developing bedside rapid diagnosticsen_UK
dc.relation.funderrefNBen_UK
Appears in Collections:Aquaculture Journal Articles

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