Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31286
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Mobile laboratory reveals the circulation of Dengue virus serotype I of Asian origin in Medina Gounass (Guediawaye), Senegal
Author(s): Dieng, Idrissa
Hedible, Boris Gildas
Diagne, Moussa Moïse
El Wahed, Ahmed Abd
Diagne, Cheikh Tidiane
Fall, Cheikh
Richard, Vincent
Vray, Muriel
Weidmann, Manfred
Faye, Ousmane
Sall, Amadou Alpha
Faye, Oumar
Keywords: Fever
NMFI
Mobile laboratory
RPA
DENV
Issue Date: Jun-2020
Citation: Dieng I, Hedible BG, Diagne MM, El Wahed AA, Diagne CT, Fall C, Richard V, Vray M, Weidmann M, Faye O, Sall AA & Faye O (2020) Mobile laboratory reveals the circulation of Dengue virus serotype I of Asian origin in Medina Gounass (Guediawaye), Senegal. Diagnostics, 10 (6), Art. No.: 408. https://doi.org/10.3390/diagnostics10060408
Abstract: With the growing success of controlling malaria in Sub-Saharan Africa, the incidence of fever due to malaria is in decline, whereas the proportion of patients with non-malaria febrile illness (NMFI) is increasing. Clinical diagnosis of NMFI is hampered by unspecific symptoms but early diagnosis is a key factor for better patient care and for disease control. The aim of this study was to determine the arboviral aetiologies of NMFI in a low resources settings using a mobile laboratory based on Recombinase Polymerase Amplification (RPA) assays. The panel of tests for this study was expanded to five arboviruses including (Dengue virus (DENV), Zika virus (ZIKV), Yellow fever virus (YFV), Chikungunya virus (CHIKV), and Rift valley fever virus (RVFV). One hundred and four children aged between one month and 115 months were enrolled and screened. Three of 104 blood samples of children < 10 years presenting at an outpatient clinic tested positive for DENV. The results were confirmed by real-time RT-PCR, partial sequencing, and non structural protein 1 (NS1) antigen capture ELISA (Biorad, France). Phylogenetic analysis of the derived DENV-1 sequences clustered them with sequences of DENV-1 isolates from Guangzhou in China in 2014. In conclusion, this mobile setup proved reliable for the rapid identification of the causative agent of NMFI, with results consistent with those obtained in the reference laboratory`s settings.
DOI Link: 10.3390/diagnostics10060408
Rights: © 2020 by the authors. Submitted for possible open access publication under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Licence URL(s): http://creativecommons.org/licenses/by/4.0/

Files in This Item:
File Description SizeFormat 
diagnostics-10-00408.pdfFulltext - Published Version2.97 MBAdobe PDFView/Open



This item is protected by original copyright



A file in this item is licensed under a Creative Commons License Creative Commons

Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.