Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31164
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dc.contributor.authorMetselaar, Matthijsen_UK
dc.contributor.authorThompson, Kim Den_UK
dc.contributor.authorPaley, Richarden_UK
dc.contributor.authorGreen, Darren Men_UK
dc.contributor.authorVerner-Jeffreys, Daviden_UK
dc.contributor.authorFeist, Stephenen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.date.accessioned2020-05-19T00:05:30Z-
dc.date.available2020-05-19T00:05:30Z-
dc.date.issued2020-11-15en_UK
dc.identifier.other735485en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31164-
dc.description.abstractRed mark syndrome (RMS) is a skin condition in Rainbow trout Oncorhynchus mykiss that has been reported worldwide but was first seen in the United Kingdom (UK) in 2003. The current study was conducted to examine if there was an association between a Midichloria-like organism (MLO) and RMS using a statistically appropriate sample set, whilst determining if there is a lack of association with Flavobacterium psychrophilum implicated in disease in previous studies. Fish in this study were obtained from three sites positive for RMS in the UK and United States (US), and three sites in the UK and the Netherlands that had no previous history of this condition. Samples taken from RMS-affected sites were found to show typical RMS pathology. Analysis of the major organs of affected fish by quantitative polymerase chain reaction (qPCR) demonstrated a significantly higher presence of the MLO in the RMS-affected tissues. Although most of the tissues were positive for the MLO, the highest correlation was seen in the skin, whilst the tissues from the unaffected fish were all negative. Thus, a strong positive correlation was found between the MLO and RMS-affected fish, whilst no association was found between the RMS-affected fish and F. psychrophilum other than superficial presence in the skin. The use of immunohistochemistry showed positive staining of what was considered to be MLO-related antigens in the internal organs of most RMS-affected fish. Attempts were made to culture the MLO, but no MLO was isolated.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationMetselaar M, Thompson KD, Paley R, Green DM, Verner-Jeffreys D, Feist S & Adams A (2020) Investigating the involvement of a Midichloria -like organism (MLO) in red mark syndrome in rainbow trout Oncorhynchus mykiss. Aquaculture, 528, Art. No.: 735485. https://doi.org/10.1016/j.aquaculture.2020.735485en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Metselaar M, Thompson KD, Paley R, Green DM, Verner-Jeffreys D, Feist S & Adams A (2020) Investigating the involvement of a Midichloria -like organism (MLO) in red mark syndrome in rainbow trout Oncorhynchus mykiss. Aquaculture, 528, Art. No.: 735485. https://doi.org/10.1016/j.aquaculture.2020.735485 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectRed mark syndromeen_UK
dc.subjectOncorhynchus mykissen_UK
dc.subjectMidichloria -like organismen_UK
dc.subjectDisease correlationen_UK
dc.subjectEpidemiologyen_UK
dc.subjectImmunohistochemistryen_UK
dc.subjectQuantitative PCRen_UK
dc.titleInvestigating the involvement of a Midichloria -like organism (MLO) in red mark syndrome in rainbow trout Oncorhynchus mykissen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2021-05-18en_UK
dc.rights.embargoreason[AQUA_2018_2613 revised manuscript 3rd submission.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2020.735485en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume528en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderUniversity of Stirling and Intervet-Schering Ploughen_UK
dc.author.emaildarren.green@stir.ac.uken_UK
dc.citation.date17/05/2020en_UK
dc.contributor.affiliationBenchmark Animal Healthen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationCEFAS - Centre for Environment, Fisheries and Aquaculture Scienceen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCEFAS - Centre for Environment, Fisheries and Aquaculture Scienceen_UK
dc.contributor.affiliationCEFAS - Centre for Environment, Fisheries and Aquaculture Scienceen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000553682000020en_UK
dc.identifier.scopusid2-s2.0-85085331629en_UK
dc.identifier.wtid1618856en_UK
dc.contributor.orcid0000-0001-9026-5675en_UK
dc.date.accepted2020-04-29en_UK
dcterms.dateAccepted2020-04-29en_UK
dc.date.filedepositdate2020-05-18en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorMetselaar, Matthijs|en_UK
local.rioxx.authorThompson, Kim D|en_UK
local.rioxx.authorPaley, Richard|en_UK
local.rioxx.authorGreen, Darren M|0000-0001-9026-5675en_UK
local.rioxx.authorVerner-Jeffreys, David|en_UK
local.rioxx.authorFeist, Stephen|en_UK
local.rioxx.authorAdams, Alexandra|en_UK
local.rioxx.projectProject ID unknown|University of Stirling and Intervet-Schering Plough|en_UK
local.rioxx.freetoreaddate2021-05-18en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2021-05-17en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2021-05-18|en_UK
local.rioxx.filenameAQUA_2018_2613 revised manuscript 3rd submission.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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