Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/31113
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dc.contributor.authorPalma, Marianaen_UK
dc.contributor.authorTrenkner, Lauren Hen_UK
dc.contributor.authorRito, Joaoen_UK
dc.contributor.authorTavares, Ludgero Cen_UK
dc.contributor.authorSilva, Emanuelen_UK
dc.contributor.authorGlencross, Brett Den_UK
dc.contributor.authorJones, John Gen_UK
dc.contributor.authorWade, Nicholas Men_UK
dc.contributor.authorViegas, Ivanen_UK
dc.date.accessioned2020-05-09T00:00:52Z-
dc.date.available2020-05-09T00:00:52Z-
dc.date.issued2020en_UK
dc.identifier.other205en_UK
dc.identifier.urihttp://hdl.handle.net/1893/31113-
dc.description.abstractPractical diets for commercial barramundi production rarely contain greater than 10% starch, used mainly as a binding agent during extrusion. Alternative ingredients such as digestible starch have shown some capacity to spare dietary protein catabolism to generate glucose. In the present study, a carnivorous fish species, the Asian seabass (Lates calcarifer) was subjected to two diets with the same digestible energy: Protein (P) – with high protein content (no digestible starch); and Starch (S) – with high digestible (pregelatinized) starch content. The effects of a high starch content diet on hepatic glycogen synthesis as well as the muscle and liver metabolome were studied using a complementary approach of 1H and 2H NMR. The hepatosomatic index was lower for fish fed high starch content diet while the concentration of hepatic glycogen was similar between groups. However, increased glycogen synthesis via the direct pathway was observed in the fish fed high starch content diet which is indicative of increased carbohydrate utilization. Multivariate analysis also showed differences between groups in the metabolome of both tissues. Univariate analysis revealed more variations in liver than in muscle of fish fed high starch content diet. Variations in metabolome were generally in agreement with the increase in the glycogen synthesis through direct pathway, however, this metabolic shift seemed to be insufficient to keep the growth rate as ensured by the diet with high protein content. Although liver glycogen does not make up a substantial quantity of total stored dietary energy in carnivorous fish, it is a key regulatory intermediate in dietary energy utilization.en_UK
dc.language.isoenen_UK
dc.publisherFrontiers Mediaen_UK
dc.relationPalma M, Trenkner LH, Rito J, Tavares LC, Silva E, Glencross BD, Jones JG, Wade NM & Viegas I (2020) Limitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed by NMR-Based Metabolomics. Frontiers in Physiology, 11, Art. No.: 205. https://doi.org/10.3389/fphys.2020.00205en_UK
dc.rights© 2020 Palma, Trenkner, Rito, Tavares, Silva, Glencross, Jones, Wade and Viegas. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY - https://creativecommons.org/licenses/by/4.0/). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectAsian seabassen_UK
dc.subject2H NMRen_UK
dc.subjectmetabolomicsen_UK
dc.subjectaquacultureen_UK
dc.subjecthepatic glycogenen_UK
dc.titleLimitations to Starch Utilization in Barramundi (Lates calcarifer) as Revealed by NMR-Based Metabolomicsen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.3389/fphys.2020.00205en_UK
dc.identifier.pmid32265728en_UK
dc.citation.jtitleFrontiers in Physiologyen_UK
dc.citation.issn1664-042Xen_UK
dc.citation.volume11en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderCommonwealth Scientific Industrial Research Organisationen_UK
dc.citation.date20/03/2020en_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Queenslanden_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.contributor.affiliationCSIRO Agriculture and Fooden_UK
dc.contributor.affiliationUniversity of Coimbraen_UK
dc.identifier.isiWOS:000525531100001en_UK
dc.identifier.scopusid2-s2.0-85083048173en_UK
dc.identifier.wtid1613387en_UK
dc.contributor.orcid0000-0003-1167-8530en_UK
dc.date.accepted2020-02-21en_UK
dcterms.dateAccepted2020-02-21en_UK
dc.date.filedepositdate2020-05-08en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorPalma, Mariana|en_UK
local.rioxx.authorTrenkner, Lauren H|en_UK
local.rioxx.authorRito, Joao|en_UK
local.rioxx.authorTavares, Ludgero C|en_UK
local.rioxx.authorSilva, Emanuel|en_UK
local.rioxx.authorGlencross, Brett D|0000-0003-1167-8530en_UK
local.rioxx.authorJones, John G|en_UK
local.rioxx.authorWade, Nicholas M|en_UK
local.rioxx.authorViegas, Ivan|en_UK
local.rioxx.projectProject ID unknown|Commonwealth Scientific Industrial Research Organisation|en_UK
local.rioxx.freetoreaddate2020-05-08en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2020-05-08|en_UK
local.rioxx.filenamefphys-11-00205.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1664-042Xen_UK
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