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Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/3104

Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Genotype-specific responses in Atlantic salmon (Salmo salar) subject to dietary fish oil replacement by vegetable oil: a liver transcriptomic analysis
Author(s): Morais, Sofia
Pratoomyot, Jarunan
Taggart, John
Bron, James
Guy, Derrick R
Bell, J Gordon
Tocher, Douglas R
Contact Email: drt1@stir.ac.uk
Keywords: Atlantic salmon
Genotype
fish oil
vegetable oil
liver
transcriptome
microarray
metabolism
Issue Date: 20-May-2011
Publisher: BioMed Central Ltd
Citation: Morais S, Pratoomyot J, Taggart J, Bron J, Guy DR, Bell JG & Tocher DR (2011) Genotype-specific responses in Atlantic salmon (Salmo salar) subject to dietary fish oil replacement by vegetable oil: a liver transcriptomic analysis, BMC Genomics, 12, p. 255.
Abstract: Background: Expansion of aquaculture is seriously limited by reductions in fish oil (FO) supply for aquafeeds. Terrestrial alternatives such as vegetable oils (VO) have been investigated and recently a strategy combining genetic selection with changes in diet formulations has been proposed to meet growing demands for aquaculture products. This study investigates the influence of genotype on transcriptomic responses to sustainable feeds in Atlantic salmon. Results: A microarray analysis was performed to investigate the liver transcriptome of two family groups selected according to their estimated breeding values (EBVs) for flesh lipid content, 'Lean' or 'Fat', fed diets containing either FO or a VO blend. Diet principally affected metabolism genes, mainly of lipid and carbohydrate, followed by immune response genes. Genotype had a much lower impact on metabolism-related genes and affected mostly signalling pathways. Replacement of dietary FO by VO caused an up-regulation of long-chain polyunsaturated fatty acid biosynthesis, but there was a clear genotype effect as fatty acyl elongase (elovl2) was only up-regulated and desaturases (D5fad and D6fad) showed a higher magnitude of response in Lean fish, which was reflected in liver fatty acid composition. Fatty acid synthase (FAS) was also up-regulated by VO and the effect was independent of genotype. Genetic background of the fish clearly affected regulation of lipid metabolism, as PPARα and PPARβ were down-regulated by the VO diet only in Lean fish, while in Fat salmon SREBP-1 expression was up-regulated by VO. In addition, all three genes had a lower expression in the Lean family group than in the Fat, when fed VO. Differences in muscle adiposity between family groups may have been caused by higher levels of hepatic fatty acid and glycerophospholipid synthesis in the Fat fish, as indicated by the expression of FAS, 1- acyl-sn-glycerol-3-phosphate acyltransferase and lipid phosphate phosphohydrolase 2. Conclusions: This study has identified metabolic pathways and key regulators that may respond differently to alternative plant-based feeds depending on genotype. Further studies are required but data suggest that it will be possible to identify families better adapted to alternative diet formulations that might be appropriate for future genetic selection programmes.
Type: Journal Article
URI: http://hdl.handle.net/1893/3104
URL: http://www.biomedcentral.com/bmcgenomics/12
DOI Link: http://dx.doi.org/10.1186/1471-2164-12-255
Rights: BMC Genomics 2011, 12:255 doi:10.1186/1471-2164-12-255; © 2011 Morais et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Affiliation: Aquaculture
University of Stirling
Aquaculture
Aquaculture
Landcatch Natural Selection Ltd
Aquaculture
Aquaculture

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