Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30905
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Reclassification of Francisella noatunensis subsp. orientalis Ottem et al. 2009 as Francisella orientalis sp. nov., Francisella noatunensis subsp. chilensis subsp. nov. and emended description of Francisella noatunensis
Author(s): Ramirez-Paredes, Jose Gustavo
Larsson, Par
Thompson, Kim D
Penman, David J
Busse, Hans-Jurgen
Ohrman, Caroline
Sjodin, Andreas
Soto, Esteban
Richards, Randolph H
Adams, Alexandra
Colquhoun, Duncan J
Keywords: Francisella orientalis
Francisella salmon Chile
Francisella new species
Francisella noatunensis subsp. chilensis
tilapia diseases and Francisella tilapia
Issue Date: 2020
Citation: Ramirez-Paredes JG, Larsson P, Thompson KD, Penman DJ, Busse H, Ohrman C, Sjodin A, Soto E, Richards RH, Adams A & Colquhoun DJ (2020) Reclassification of Francisella noatunensis subsp. orientalis Ottem et al. 2009 as Francisella orientalis sp. nov., Francisella noatunensis subsp. chilensis subsp. nov. and emended description of Francisella noatunensis. International Journal of Systematic and Evolutionary Microbiology, 70 (3), p. 2034–2048. https://doi.org/10.1099/ijsem.0.004009
Abstract: Francisella noatunensis is a fastidious facultative intracellular bacterial pathogen that causes ‘piscine francisellosis’, a serious disease affecting both marine and fresh water farmed and wild fish worldwide. Currently two F. noatunensis subspecies are recognized, i.e. F. noatunensis subsp. noatunensis and F. noatunensis subsp. orientalis . In the present study, the taxonomy of F. noatunensis was revisited using a polyphasic approach, including whole genome derived parameters such as digital DNA–DNA hybridization, whole genome average nucleotide identity (wg-ANIm), whole genome phylogenetic analysis, whole genome G+C content, metabolic fingerprinting and chemotaxonomic analyses. The results indicated that isolates belonging to F. noatunensis subsp. orientalis represent a phenotypically and genetically homogenous taxon, clearly distinguishable from F. noatunensis subsp. noatunensis that fulfils requirements for separate species status. We propose, therefore, elevation of F. noatunensis subsp. orientalis to the species rank as Francisella orientalis sp. nov. with the type strain remaining as Ehime-1T (DSM 21254T=LMG 24544T). Furthermore, we identified sufficient phenotypic and genetic differences between F. noatunensis subsp. noatunensis recovered from diseased farmed Atlantic salmon in Chile and those isolated from wild and farmed Atlantic cod in Northern Europe to warrant proposal of the Chilean as a novel F. noatunensis subspecies, i.e. Francisella noatunensis subsp. chilensis subsp. nov. with strain PQ1106T (CECT 9798T=NCTC14375T) as the type strain. Finally, we emend the description of F. noatunensis by including further metabolic information and the description of atypical strains.
DOI Link: 10.1099/ijsem.0.004009
Rights: © Ramirez-Paredes JG, Larsson P, Thompson KD, Penman DJ, Busse H, Ohrman C, Sjodin A, Soto E, Richards RH, Adams A & Colquhoun DJ, 2020. The definitive peer reviewed, edited version of this article is published in International Journal of Systematic and Evolutionary Microbiology, 2020, https://doi.org/10.1099/ijsem.0.004009
Licence URL(s): http://creativecommons.org/licenses/by/4.0/

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