Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30887
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dc.contributor.authorAmbuAli, Aishaen_UK
dc.contributor.authorMonaghan, Sean Jen_UK
dc.contributor.authorMcLean, Kevinen_UK
dc.contributor.authorInglis, Neil Fen_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorWehner, Stefanieen_UK
dc.contributor.authorBron, James Een_UK
dc.date.accessioned2020-03-31T00:04:42Z-
dc.date.available2020-03-31T00:04:42Z-
dc.date.issued2020en_UK
dc.identifier.other88en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30887-
dc.description.abstractBackground It is hypothesised that being a blood-feeding ectoparasite, Argulus foliaceus (Linnaeus, 1758), uses similar mechanisms for digestion and host immune evasion to those used by other haematophagous ecdysozoa, including caligid copepods (e.g. sea louse). We recently described and characterised glands associated with the feeding appendages of A. foliaceus using histological techniques. The work described in the present study is the first undertaken with the objective of identifying and partially characterising the components secreted from these glands using a proteomic approach. Methods Argulus foliaceus parasites were sampled from the skin of rainbow trout (Oncorhynchus mykiss), from Loch Fad on the Isle of Bute, Scotland, UK. The proteins from A. foliaceus secretory/excretory products (SEPs) were collected from the supernatant of artificial freshwater conditioned with active adult parasites (n = 5–9 per ml; n = 560 total). Proteins within the SEPs were identified and characterised using LC-ESI-MS/MS analysis. Data are available via ProteomeXchange with identifier PXD016226. Results Data mining of a protein database translated from an A. foliaceus dataset using ProteinScape allowed identification of 27 predicted protein sequences from the A. foliaceus SEPs, each protein matching the criteria of 2 peptides with at least 4 contiguous amino acids. Nine proteins had no matching sequence through OmicsBox (Blast2GO) analysis searches suggesting that Argulus spp. may additionally have unique proteins present in their SEPs. SignalP 5.0 software, identified 13 proteins with a signal sequence suggestive of signal peptides and supportive of secreted proteins being identified. Notably, the functional characteristics of identified A. foliaceus proteins/domains have also been described from the salivary glands and saliva of other blood-feeding arthropods such as ticks. Identified proteins included: transporters, peroxidases, metalloproteases, proteases and serine protease inhibitors which are known to play roles in parasite immune evasion/induction (e.g. astacin), immunomodulation (e.g. serpin) and digestion (e.g. trypsin). Conclusions To our knowledge, the present study represents the first proteomic analysis undertaken for SEPs from any branchiuran fish louse. Here we reveal possible functional roles of A. foliaceus SEPs in digestion and immunomodulation, with a number of protein families shared with other haematophagous ectoparasites. A number of apparently unique secreted proteins were identified compared to other haematophagous ecdysozoa.en_UK
dc.language.isoenen_UK
dc.publisherSpringer Science and Business Media LLCen_UK
dc.relationAmbuAli A, Monaghan SJ, McLean K, Inglis NF, Bekaert M, Wehner S & Bron JE (2020) Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa. Parasites and Vectors, 13 (1), Art. No.: 88. https://doi.org/10.1186/s13071-020-3964-zen_UK
dc.rightsThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectSecretionsen_UK
dc.subjectImmunomodulationen_UK
dc.subjectFish liceen_UK
dc.subjectArgulusen_UK
dc.subjectBranchiuraen_UK
dc.titleIdentification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoaen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/s13071-020-3964-zen_UK
dc.identifier.pmid32070416en_UK
dc.citation.jtitleParasites and Vectorsen_UK
dc.citation.issn1756-3305en_UK
dc.citation.issn1756-3305en_UK
dc.citation.volume13en_UK
dc.citation.issue1en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderSultan Qaboos Universityen_UK
dc.citation.date18/02/2020en_UK
dc.contributor.affiliationSultan Qaboos Universityen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationThe Moredun Research Instituteen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationMax Planck Institute of Psychiatryen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000517227400005en_UK
dc.identifier.scopusid2-s2.0-85079794730en_UK
dc.identifier.wtid1575289en_UK
dc.contributor.orcid0000-0002-6967-8915en_UK
dc.contributor.orcid0000-0002-7692-7756en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-3632-2584en_UK
dc.contributor.orcid0000-0003-3544-0519en_UK
dc.date.accepted2020-02-13en_UK
dcterms.dateAccepted2020-02-13en_UK
dc.date.filedepositdate2020-03-30en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionVoRen_UK
local.rioxx.authorAmbuAli, Aisha|0000-0002-6967-8915en_UK
local.rioxx.authorMonaghan, Sean J|0000-0002-7692-7756en_UK
local.rioxx.authorMcLean, Kevin|en_UK
local.rioxx.authorInglis, Neil F|en_UK
local.rioxx.authorBekaert, Michaël|0000-0002-1206-7654en_UK
local.rioxx.authorWehner, Stefanie|0000-0002-3632-2584en_UK
local.rioxx.authorBron, James E|0000-0003-3544-0519en_UK
local.rioxx.projectProject ID unknown|Sultan Qaboos University|en_UK
local.rioxx.freetoreaddate2020-03-30en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by/4.0/|2020-03-30|en_UK
local.rioxx.filenames13071-020-3964-z.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source1756-3305en_UK
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