Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30882
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dc.contributor.authorGarrido, Diegoen_UK
dc.contributor.authorMonroig, Oscaren_UK
dc.contributor.authorGalindo, Anaen_UK
dc.contributor.authorBetancor, Monicaen_UK
dc.contributor.authorPerez, Jose Antonioen_UK
dc.contributor.authorKabeya, Naokien_UK
dc.contributor.authorMarrero, Manuelen_UK
dc.contributor.authorRodriguez, Covadongaen_UK
dc.date.accessioned2020-03-31T00:02:45Z-
dc.date.available2020-03-31T00:02:45Z-
dc.date.issued2020-06-30en_UK
dc.identifier.other735147en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30882-
dc.description.abstractCarps, barbels and other cyprinids are the major contributors to freshwater aquaculture at global scale. Nevertheless, freshwater fish aquaculture needs to diversify their production in order to offer consumers new species. Tench (Tinca tinca) is a freshwater species with great interest for the diversification of continental aquaculture. However, up to date, no commercial formulated diet exists for this species in order to optimize their nutritional requirements and the quality of its final product. Using multiple methodological approaches, the aim of this study was to evaluate the long chain polyunsaturated fatty acid (LC-PUFA) metabolism of T. tinca. Firstly, the molecular cloning and functional characterisation by heterologous expression in yeast of a desaturase (Fads2) and two elongases (Elovl2 and Elovl5) involved in LC-PUFA biosynthesis, and the analysis of gene expression among tissues were performed. Secondly, in order to confirm the LC-PUFA biosynthesis capacity of isolated hepatocytes and enterocytes, cells were incubated with [1-14C] labelled linoleic acid (18:2n-6, LA), linolenic acid (18:3n-3, ALA) and eicosapentaenoic acid (20:5n-3, EPA). In yeast, Fads2 showed a Δ6/Δ5 bifunctional activity. Elovl2 was more active over C20 and C22 substrates, whereas Elovl5 was over C18 and C20. Liver displayed the highest expression for the three target genes (fads2, elovl2 and elovl5). Incubated cells also showed Fads2 bifunctional activity as well as elongation products in concordance with yeast heterologous expression results. Importantly, our results demonstrated that tench is able to biosynthesise docosahexaenoic acid (DHA) from 18:3n-3 in both hepatocytes and enterocytes, a capacity that seems to explain in part the surprisingly high levels of DHA found in the fish flesh compared to its dietary supply. Tench is a promising freshwater species with a potential capacity to endogenously increase its flesh DHA contents, reducing the impact that the usage of fish oils from forage fisheries may have on the aquaculture industry.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationGarrido D, Monroig O, Galindo A, Betancor M, Perez JA, Kabeya N, Marrero M & Rodriguez C (2020) Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity. Aquaculture, 523, Art. No.: 735147. https://doi.org/10.1016/j.aquaculture.2020.735147en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Garrido D, Monroig O, Galindo A, Betancor M, Perez JA, Kabeya N, Marrero M & Rodriguez C (2020) Lipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacity. Aquaculture, 523, Art. No.: 735147. DOI: https://doi.org/10.1016/j.aquaculture.2020.735147 © 2020, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectBiosynthesisen_UK
dc.subjectFatty acyl desaturaseen_UK
dc.subjectFatty acyl elongaseen_UK
dc.subjectLong-chain polyunsaturated fatty aciden_UK
dc.subjectRadiolabelled fatty aciden_UK
dc.subjectTinca tincaen_UK
dc.titleLipid metabolism in Tinca tinca and its n-3 LC-PUFA biosynthesis capacityen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2021-02-22en_UK
dc.rights.embargoreason[Garrido et al 2020.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.aquaculture.2020.735147en_UK
dc.citation.jtitleAquacultureen_UK
dc.citation.issn0044-8486en_UK
dc.citation.volume523en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderMinistry of Economy and Competitiveness (Spain)en_UK
dc.author.emailm.b.betancor@stir.ac.uken_UK
dc.citation.date21/02/2020en_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquaculture Torre de la Salen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of Tokyoen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.contributor.affiliationUniversity of La Lagunaen_UK
dc.identifier.isiWOS:000526415400008en_UK
dc.identifier.scopusid2-s2.0-85080035909en_UK
dc.identifier.wtid1573976en_UK
dc.contributor.orcid0000-0003-1626-7458en_UK
dc.date.accepted2020-02-20en_UK
dcterms.dateAccepted2020-02-20en_UK
dc.date.filedepositdate2020-02-21en_UK
rioxxterms.apcnot requireden_UK
rioxxterms.typeJournal Article/Reviewen_UK
rioxxterms.versionAMen_UK
local.rioxx.authorGarrido, Diego|en_UK
local.rioxx.authorMonroig, Oscar|en_UK
local.rioxx.authorGalindo, Ana|en_UK
local.rioxx.authorBetancor, Monica|0000-0003-1626-7458en_UK
local.rioxx.authorPerez, Jose Antonio|en_UK
local.rioxx.authorKabeya, Naoki|en_UK
local.rioxx.authorMarrero, Manuel|en_UK
local.rioxx.authorRodriguez, Covadonga|en_UK
local.rioxx.projectProject ID unknown|Ministry of Economy and Competitiveness (Spain)|en_UK
local.rioxx.freetoreaddate2021-02-22en_UK
local.rioxx.licencehttp://www.rioxx.net/licenses/under-embargo-all-rights-reserved||2021-02-21en_UK
local.rioxx.licencehttp://creativecommons.org/licenses/by-nc-nd/4.0/|2021-02-22|en_UK
local.rioxx.filenameGarrido et al 2020.pdfen_UK
local.rioxx.filecount1en_UK
local.rioxx.source0044-8486en_UK
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