Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30763
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dc.contributor.authorDjainal, Winarti Achmad Sarminen_UK
dc.contributor.authorShahin, Khaliden_UK
dc.contributor.authorMetselaar, Matthijsen_UK
dc.contributor.authorAdams, Alexandraen_UK
dc.contributor.authorDesbois, Andrew Pen_UK
dc.date.accessioned2020-02-29T01:18:20Z-
dc.date.available2020-02-29T01:18:20Z-
dc.date.issued2020en_UK
dc.identifier.other8en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30763-
dc.description.abstractBackground Francisella noatunensis subsp. orientalis (Fno) is the etiological agent of francisellosis in cultured warm water fish, such as tilapia. Antibiotics are administered to treat the disease but a better understanding of Fno infection biology will inform improved treatment and prevention measures. However, studies with native hosts are costly and considerable benefits would derive from access to a practical alternative host. Here, larvae of Galleria mellonella were assessed for suitability to study Fno virulence. Results Larvae were killed by Fno in a dose-dependent manner but the insects could be rescued from lethal doses of bacteria by antibiotic therapy. Infection progression was assessed by histopathology (haematoxylin and eosin staining, Gram Twort and immunohistochemistry) and enumeration of bacteria recovered from the larval haemolymph on selective agar. Fno was phagocytosed and could survive intracellularly, which is consistent with observations in fish. Virulence of five Fno isolates showed strong agreement between G. mellonella and red Nile tilapia hosts. Conclusions This study shows that an alternative host, G. mellonella, can be applied to understand Fno infections, which will assist efforts to identify solutions to piscine francisellosis thus securing the livelihoods of tilapia farmers worldwide and ensuring the production of this important food source.en_UK
dc.language.isoenen_UK
dc.publisherBMCen_UK
dc.relationDjainal WAS, Shahin K, Metselaar M, Adams A & Desbois AP (2020) Larva of greater wax moth Galleria mellonella is a suitable alternative host for the fish pathogen Francisella noatunensis subsp. orientalis. BMC Microbiology, 20, Art. No.: 8. https://doi.org/10.1186/s12866-020-1695-0en_UK
dc.rightsThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.subjectAlternative modelen_UK
dc.subjectAquacultureen_UK
dc.subjectPiscine francisellosisen_UK
dc.subjectOreochromis niloticusen_UK
dc.subjectRed Nile tilapiaen_UK
dc.titleLarva of greater wax moth Galleria mellonella is a suitable alternative host for the fish pathogen Francisella noatunensis subsp. orientalisen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1186/s12866-020-1695-0en_UK
dc.identifier.pmid31918661en_UK
dc.citation.jtitleBMC Microbiologyen_UK
dc.citation.issn1471-2180en_UK
dc.citation.volume20en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.contributor.funderBenchmark Animal Healthen_UK
dc.citation.date09/01/2020en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationBenchmark Animal Healthen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000521298800001en_UK
dc.identifier.scopusid2-s2.0-85077693835en_UK
dc.identifier.wtid1537936en_UK
dc.contributor.orcid0000-0002-4912-6427en_UK
dc.contributor.orcid0000-0001-6052-8761en_UK
dc.date.accepted2019-12-31en_UK
dc.date.filedepositdate2020-01-30en_UK
Appears in Collections:Aquaculture Journal Articles

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