Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30717
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Thermal and temporal stability on the enteric viruses infectivity in surface freshwater
Author(s): Moresco, Vanessa
Damazo, Natalia Alzira
Barardi, Celia Regina Monte
Contact Email: vanessa.moresco@stir.ac.uk
Keywords: enteric viruses
freshwater
infectivity
storage temperature
Issue Date: Jun-2016
Citation: Moresco V, Damazo NA & Barardi CRM (2016) Thermal and temporal stability on the enteric viruses infectivity in surface freshwater. Water Science and Technology: Water Supply, 16 (3), pp. 620-627. https://doi.org/10.2166/ws.2015.171
Abstract: The present study aimed to evaluate the stability of Human Adenovirus type 2 (HAdV2) and Murine Norovirus 1 (MNV-1) in surface freshwater samples stored at different temperatures. For HAdV2 the stability decreased with increasing temperatures (−80 > −20 > 4 > 22 °C). The time required to reach one log reduction in viral titers (T90) was similar among all the times and temperatures by different cell-culture based methods and reverse transcription-quantitative polymerase chain reaction (RT-qPCR). The HAdV2 stability decreased with the time of storage temperature and methods employed, aside from samples stored at 22 and 4 °C which showed the lowest T90 values (50 days). For MNV-1, the samples stored at 22 and −20 °C showed higher log10 decay values, followed by 4 and −80 °C; while genome persistence was ranked as −80 > −20 > 4 > 22 °C. The T90 values were lower for samples stored at 22 °C (33 days), followed by 4, −20 and −80 °C with 111, 100 and 333 days, respectively. The results indicate that, under laboratory storage conditions, freshwater samples should be kept at 4 °C and at −80 °C for short- and long-term periods, respectively. This study provided useful information about thermal and temporal stability of the enteric viruses regarding sample storage conditions.
DOI Link: 10.2166/ws.2015.171
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.

Files in This Item:
File Description SizeFormat 
Moresco-etal-WSTWS-2016.pdfFulltext - Published Version273.68 kBAdobe PDFUnder Permanent Embargo    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.