Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30618
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dc.contributor.authorOliveira, Catarina C Ven_UK
dc.contributor.authorMcStay, Elsbethen_UK
dc.contributor.authorCabrita, Elsaen_UK
dc.contributor.authorCastanheira, Maria Filipaen_UK
dc.contributor.authorMigaud, Hervéen_UK
dc.contributor.authorDinis, Maria Teresaen_UK
dc.contributor.authorDavie, Andrewen_UK
dc.date.accessioned2020-01-14T01:03:26Z-
dc.date.available2020-01-14T01:03:26Z-
dc.date.issued2020-03en_UK
dc.identifier.other110624en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30618-
dc.description.abstractThe kisspeptin system, a known regulator of reproduction in fish, was investigated during two key phases within the gilthead seabream (Sparus aurata) life cycle: protandrous sex change and larval ontogeny. Seabream specific partial cDNA sequences were identified for two key targets, kissr4 and kiss2, which were subsequently cloned and qPCR assays developed. Thereafter, to examine association in expression with sex change, a group of adult seabream (2+ years old) undergoing sex change were sampled for gene expression at two different periods of the annual cycle. To study the kisspeptin system ontogeny during early life stages, transcript levels were monitored in larvae (till 30 days-post-hatch, DPH) and post-larvae (from 30 till 140 DPH). During sex change, higher expression of kissr4 and kiss2 was observed in males when compared to females or individual undergoing sex change, this is suggestive of differential actions of the kisspeptin system during protandrous sex change. Equally, variable expression of the kisspeptin system during early ontogenic development was observed. The higher expression of kissr4 and kiss2 observed from 5 DPH, with elevations at 5–20 and 90 DPH for kissr4 and at 5, 10, 20, and 60 DPH for kiss2, is coincident with the early ontogeny of gnrh genes previously reported for seabream, and possibly related with early development of the reproductive axis in this species.en_UK
dc.language.isoenen_UK
dc.publisherElsevier BVen_UK
dc.relationOliveira CCV, McStay E, Cabrita E, Castanheira MF, Migaud H, Dinis MT & Davie A (2020) Investigating the kisspeptin system in the hermaphrodite teleost gilthead seabream (Sparus aurata). Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 241, Art. No.: 110624. https://doi.org/10.1016/j.cbpa.2019.110624en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Oliveira CCV, McStay E, Cabrita E, Castanheira MF, Migaud H, Dinis MT & Davie A (2020) Investigating the kisspeptin system in the hermaphrodite teleost gilthead seabream (Sparus aurata). Comparative Biochemistry and Physiology Part A: Molecular and Integrative Physiology, 241, Art. No.: 110624 DOI: https://doi.org/10.1016/j.cbpa.2019.110624 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectSex changeen_UK
dc.subjectkissr4en_UK
dc.subjectkiss2en_UK
dc.subjectProtandric hermaphroditisen_UK
dc.subjectmontogenyen_UK
dc.titleInvestigating the kisspeptin system in the hermaphrodite teleost gilthead seabream (Sparus aurata)en_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-12-07en_UK
dc.rights.embargoreason[Oliveira et al_kiss seabream_CBP_revision.pdf] Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.cbpa.2019.110624en_UK
dc.identifier.pmid31812674en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part A: Molecular and Integrative Physiologyen_UK
dc.citation.issnComparative Biochemistry and Physiology Part A: Molecular & Integrative Physiologyen_UK
dc.citation.issn1095-6433en_UK
dc.citation.volume241en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderEuropean Community's Seventh Framework Programmeen_UK
dc.contributor.funderPortuguese Foundation for Science and Technologyen_UK
dc.author.emailandrew.davie@stir.ac.uken_UK
dc.citation.date06/12/2019en_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000514244900005en_UK
dc.identifier.scopusid2-s2.0-85076359572en_UK
dc.identifier.wtid1498230en_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.contributor.orcid0000-0002-9524-618Xen_UK
dc.date.accepted2019-11-24en_UK
dc.date.filedepositdate2020-01-06en_UK
Appears in Collections:Aquaculture Journal Articles

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