|Appears in Collections:||Computing Science and Mathematics Journal Articles|
|Peer Review Status:||Refereed|
|Title:||Predicting the minimum liquid surface tension activity of pseudomonads expressing biosurfactants|
|Author(s):||Mohammed, Ibrahim U|
Hapca, Simona M
Spiers, Andrew J
liquid surface tension
|Citation:||Mohammed IU, Deeni Y, Hapca SM, McLaughlin K & Spiers AJ (2015) Predicting the minimum liquid surface tension activity of pseudomonads expressing biosurfactants. Letters in Applied Microbiology, 60 (1), pp. 37-43. https://doi.org/10.1111/lam.12331|
|Abstract:||Bacteria produce a variety of biosurfactants capable of significantly reducing liquid (aqueous) surface tension (γ) with a range of biological roles and biotechnological uses. To determine the lowest achievable surface tension (γMin), we tested a diverse collection of Pseudomonas‐like isolates from contaminated soil and activated sludge and identified those expressing biosurfactants by drop‐collapse assay. Liquid surface tension‐reducing ability was quantitatively determined by tensiometry, with 57 isolates found to significantly lower culture supernatant surface tensions to 24·5–49·1 mN m−1. Differences in biosurfactant behaviour determined by foaming, emulsion and oil‐displacement assays were also observed amongst isolates producing surface tensions of 25–27 mN m−1, suggesting that a range of structurally diverse biosurfactants were being expressed. Individual distribution identification (IDI) analysis was used to identify the theoretical probability distribution that best fitted the surface tension data, which predicted a γMin of 24·24 mN m−1. This was in agreement with predictions based on earlier work of published mixed bacterial spp. data, suggesting a fundamental limit to the ability of bacterial biosurfactants to reduce surface tensions in aqueous systems. This implies a biological restriction on the synthesis and export of these agents or a physical–chemical restriction on their functioning once produced.|
|Rights:||The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.|
|Mohammed et al-LAM-2015.pdf||Fulltext - Published Version||195.7 kB||Adobe PDF||Under Permanent Embargo Request a copy|
Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.
This item is protected by original copyright
Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.
If you believe that any material held in STORRE infringes copyright, please contact email@example.com providing details and we will remove the Work from public display in STORRE and investigate your claim.