Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30395
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dc.contributor.authorFatsini, Elviraen_UK
dc.contributor.authorRey, Soniaen_UK
dc.contributor.authorIbarra-Zatarain, Zoharen_UK
dc.contributor.authorBoltaña, Sebastiánen_UK
dc.contributor.authorMackenzie, Simonen_UK
dc.contributor.authorDuncan, Neil Jen_UK
dc.date.accessioned2019-11-01T01:02:08Z-
dc.date.available2019-11-01T01:02:08Z-
dc.date.issued2020-01-01en_UK
dc.identifier.other112724en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30395-
dc.description.abstractIn fish, proactive and reactive individual stress copying styles (SCS) have been used to resolve variation in molecular expression data. Stress coping styles have been previously described in several stages of Solea senegalensis by validating for the species the use of standard behavioural screening tests. The present study aimed to link behavioural SCS tests with brain transcript abundance in early Senegalese sole juveniles in order to observe the natural variation in a molecular pathway in this species. A total of 50 juveniles were subjected to three individual behavioural (Restraining, New environment and Confinement) and one group (Risk-taking) screening tests. The fish were classified in SCS categories by applying a hierarchical cluster to the variable “Total activity” (the total activity time that the fish was moving in each individual test). Three categories were defined, proactive, intermediate and reactive sole. Six transcripts were chosen and tested, one related to basic metabolism (gapdh-2), three to feeding behaviour (per1, igf-Ia, pparß) and two to the stress response (crh-BP and hsp90aa) in 30 juveniles (10 individuals per SCS category) using rt-qPCR to observe differences in the abundance of those transcripts among SCS. Four transcripts were differentially expressed (DETs) among them. The transcript gapdh-2 showed up-regulation for proactive and intermediate SCS sole while reactive individuals showed down-regulation. Target mRNAs per1, igf-Ia and pparß, showed different levels of up-regulation for proactive and reactive fish while intermediates were highly down-regulated. Surprisingly no differences in stress related transcripts were observed. Correlations were found between variation in coping styles and variation in the abundance of mRNAs involved in important biological functions in Senegalese sole. These results are the first evidence of the relationship between the behavioural individual variation and the fluctuation in brain transcripts abundance in Senegalese sole.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationFatsini E, Rey S, Ibarra-Zatarain Z, Boltaña S, Mackenzie S & Duncan NJ (2020) Linking stress coping styles with brain mRNA abundance of selected transcripts for Senegalese sole (Solea senegalensis) juveniles. Physiology and Behavior, 213, Art. No.: 112724. https://doi.org/10.1016/j.physbeh.2019.112724en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Fatsini E, Rey S, Ibarra-Zatarain Z, Boltaña S, Mackenzie S & Duncan NJ (2020) Linking stress coping styles with brain mRNA abundance of selected transcripts for Senegalese sole (Solea senegalensis) juveniles. Physiology and Behavior, 213, Art. No.: 112724. DOI: https://doi.org/10.1016/j.physbeh.2019.112724 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectFlatfishen_UK
dc.subjectTranscriptsen_UK
dc.subjectBehavioural traitsen_UK
dc.subjectIndividual variationen_UK
dc.titleLinking stress coping styles with brain mRNA abundance of selected transcripts for Senegalese sole (Solea senegalensis) juvenilesen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-11-02en_UK
dc.rights.embargoreason[Fatsini et al_SCS_PB_accepted.pdf] Until this work is published there will be an embargo on the full text of this work. Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.physbeh.2019.112724en_UK
dc.identifier.pmid31682888en_UK
dc.citation.jtitlePhysiology and Behavioren_UK
dc.citation.issn1873-507Xen_UK
dc.citation.issn0031-9384en_UK
dc.citation.volume213en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderFundacao para a Ciencia e Tecnologiaen_UK
dc.author.emailsonia.reyplanellas@stir.ac.uken_UK
dc.citation.date01/11/2019en_UK
dc.contributor.affiliationUniversity of Algarveen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationCenter for Innovation and Technology Transfer - Mexicoen_UK
dc.contributor.affiliationUniversity of Concepcionen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationIRTA-Sant Carles de la Rapita, Spainen_UK
dc.identifier.isiWOS:000526852700018en_UK
dc.identifier.scopusid2-s2.0-85074920138en_UK
dc.identifier.wtid1474005en_UK
dc.contributor.orcid0000-0002-3406-3291en_UK
dc.contributor.orcid0000-0003-1845-6826en_UK
dc.date.accepted2019-10-25en_UK
dc.date.filedepositdate2019-10-31en_UK
Appears in Collections:Aquaculture Journal Articles

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