Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30287
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection
Author(s): Chang, Yao-Chung
Hamlin-Wright, Harry
Monaghan, Sean
Herath, Tharangani
Baily, Johanna
del Pozo, Jorge
Downes, Jamie
Preston, Andrew
Chalmers, Lynn
Jayasuriya, Nilantha
Bron, James E
Adams, Alexandra
Fridman, Sophie
Contact Email: sophie.fridman@stir.ac.uk
Keywords: ionoregulatory cells
mitochondria‐rich cell
Na+/K+‐ATPase
Neoparamoeba perurans
salmonid
transmission electron microscopy
Issue Date: Oct-2019
Date Deposited: 11-Oct-2019
Citation: Chang Y, Hamlin-Wright H, Monaghan S, Herath T, Baily J, del Pozo J, Downes J, Preston A, Chalmers L, Jayasuriya N, Bron JE, Adams A & Fridman S (2019) Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. Journal of Fish Diseases, 42 (10), pp. 1433-1446. https://doi.org/10.1111/jfd.13073
Abstract: Amoebic gill disease (AGD) is emerging as one of the most significant health challenges affecting farmed Atlantic salmon in the marine environment. It is caused by the amphizoic amoeba Neoparamoeba perurans, with infestation of gills causing severe hyperplastic lesions, compromising overall gill integrity and function. This study used histology, transmission electron microscopy (TEM), immunohistochemistry and transcript expression to relate AGD‐associated pathological changes to changes in the morphology and distribution of chloride cells (CCs) in the gills of Atlantic salmon (Salmo salar L.) showing the progression of an AGD infection. A marked reduction in numbers of immunolabelled CCs was detected, and a changing pattern in distribution and morphology was closely linked with the level of basal epithelial hyperplasia in the gill. In addition, acute degenerative ultrastructural changes to CCs at the lesion site were observed with TEM. These findings were supported by the early‐onset downregulation of Na+/K+‐ATPase transcript expression. This study provides supportive evidence that histological AGD lesion assessment was a good qualitative tool for AGD scoring and corresponded well with qPCR genomic Paramoeba perurans quantification. Ultrastructural changes induced in salmon CCs as a result of AGD are reported here for the first time.
DOI Link: 10.1111/jfd.13073
Rights: This item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. This is the peer reviewed version of the following article: Chang, Y‐C, Hamlin‐Wright, H, Monaghan, S, et al. Changes in distribution, morphology and ultrastructure of chloride cell in Atlantic salmon during an AGD infection. J Fish Dis. 2019; 42: 1433– 1446, which has been published in final form at https://doi.org/10.1111/jfd.13073. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for self-archiving.

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