Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30262
Appears in Collections:Biological and Environmental Sciences Journal Articles
Peer Review Status: Refereed
Title: Presence of enteric viruses, bioaccumulation and stability in Anomalocardia brasiliana clams (Gmelin, 1791)
Author(s): Souza, Doris Sobral Marques
Dominot, Ana Ferreira Ávila
Moresco, Vanessa
Barardi, Célia Regina Monte
Contact Email: vanessa.moresco@stir.ac.uk
Keywords: Anomalocardia brasiliana
Enteric virus
Clams
qPCR
Viral bioaccumulation
Thermal inactivation
Issue Date: 2-Feb-2018
Citation: Souza DSM, Dominot AFÁ, Moresco V & Barardi CRM (2018) Presence of enteric viruses, bioaccumulation and stability in Anomalocardia brasiliana clams (Gmelin, 1791). International Journal of Food Microbiology, 266, pp. 363-371. https://doi.org/10.1016/j.ijfoodmicro.2017.08.004
Abstract: Bivalve mollusks are filter feeders and may accumulate human pathogens in their tissues. Many studies demonstrated human diseases associated with bivalve consumption, especially oysters. Anomalocardia brasiliana clams are distributed along the Brazilian coastal area and are an exotic ingredient for some typical dishes in Brazil. Even though there are several reports describing the contamination of oysters and mussels with human pathogens, there is a lack of studies reporting contamination of A. brasiliana with human pathogens. An evaluation of natural microbiological contamination in A. brasiliana samples over a period of 18 months (November 2014 to April 2016) showed that the bacteria indices were in accordance with Brazilian regulations (E. coli < 230 MPN and Salmonella sp. absent in 25 g of meat). However, the enteric viruses evaluated were detected throughout the analysis period, with the highest result for the hepatitis A virus (HAV); followed by Rotavirus-A (RVA); Human Adenovirus (HAdV) and Norovirus GI (NoV GI). The bioaccumulation of enteric viruses by A. brasiliana during a period of 24 h was performed using NoV GI and GII, HAV, RVA and HAdV as models. Interestingly the mollusk demonstrated different uptake behaviors in relation to these viruses throughout the time period. NoV GI was the most adsorbed virus after 24 h. HAV concentration was < 1% at 3 h, but it increased to < 10% at 8 h, remaining unchanged until 12 h, and decreasing to < 3% at 24 h; HAdV reached its highest concentration at 12 h, being released by the animals and lowering to < 3% at 24 h. RVA bioaccumulation was unstable over time, reaching its highest values after 24 h (< 5%); NoV GII bioaccumulation remained < 1%. Thermal inactivation of HAdV-2 in A. brasiliana was also evaluated. After the usual gentle cooking procedure using different times (0, 1, 1.5, 3 and 5 mins), viral infectivity was evaluated using ICC-et-RT-qPCR. The temperature inside the DT remained < 80 °C over time and after 5 min of cooking the HAdV reached a decay of 90% (1 log10). The results showed a real warn to the consumers that can be exposed to infectious human viruses if they eat these clams improperly cooked. HAV was the most detected virus in these animals, which may lead to outbreaks. A. brasiliana exhibited distinct behavior in NoV GI bioaccumulation and persistence, pointing to the need for further studies about the cellular ligands used by these viruses to become attached to these clams.
DOI Link: 10.1016/j.ijfoodmicro.2017.08.004
Rights: The publisher does not allow this work to be made publicly available in this Repository. Please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study.

Files in This Item:
File Description SizeFormat 
1-s2.0-S0168160517303380-main.pdfFulltext - Published Version1.05 MBAdobe PDFUnder Permanent Embargo    Request a copy

Note: If any of the files in this item are currently embargoed, you can request a copy directly from the author by clicking the padlock icon above. However, this facility is dependent on the depositor still being contactable at their original email address.



This item is protected by original copyright



Items in the Repository are protected by copyright, with all rights reserved, unless otherwise indicated.

If you believe that any material held in STORRE infringes copyright, please contact library@stir.ac.uk providing details and we will remove the Work from public display in STORRE and investigate your claim.