Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30169
Appears in Collections:Aquaculture Journal Articles
Peer Review Status: Refereed
Title: Transcriptomic Analysis of Marine Gastropod Hemifusus tuba Provides Novel Insights into Conotoxin Genes
Author(s): Li, Ronghua
Bekaert, Michaël
Wu, Luning
Mu, Changkao
Song, Weiwei
Migaud, Herve
Wang, Chunlin
Keywords: Hemifusus tuba
transcriptome
conotoxin
simple sequence repeats
Issue Date: 10-Aug-2019
Citation: Li R, Bekaert M, Wu L, Mu C, Song W, Migaud H & Wang C (2019) Transcriptomic Analysis of Marine Gastropod Hemifusus tuba Provides Novel Insights into Conotoxin Genes. Marine Drugs, 17 (8), Art. No.: 466. https://doi.org/10.3390/md17080466
Abstract: The marine gastropod Hemifusus tuba is served as a luxury food in Asian countries and used in traditional Chinese medicine to treat lumbago and deafness. The lack of genomic data on H. tuba is a barrier to aquaculture development and functional characteristics of potential bioactive molecules are poorly understood. In the present study, we used high-throughput sequencing technologies to generate the first transcriptomic database of H. tuba. A total of 41 unique conopeptides were retrieved from 44 unigenes, containing 6-cysteine frameworks belonging to four superfamilies. Duplication of mature regions and alternative splicing were also found in some of the conopeptides, and the de novo assembly identified a total of 76,306 transcripts with an average length of 824.6 nt, of which including 75,620 (99.1%) were annotated. In addition, simple sequence repeats (SSRs) detection identified 14,000 unigenes containing 20,735 SSRs, among which, 23 polymorphic SSRs were screened. Thirteen of these markers could be amplified in Hemifusus ternatanus and seven in Rapana venosa. This study provides reports of conopeptide genes in Buccinidae for the first time as well as genomic resources for further drug development, gene discovery and population resource studies of this species.
DOI Link: 10.3390/md17080466
Rights: © 2019 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
Licence URL(s): http://creativecommons.org/licenses/by/4.0/

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