Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30102
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dc.contributor.authorSyaifudin, Mochamaden_UK
dc.contributor.authorBekaert, Michaëlen_UK
dc.contributor.authorTaggart, John Ben_UK
dc.contributor.authorBartie, Kerry Len_UK
dc.contributor.authorWehner, Stefanieen_UK
dc.contributor.authorPalaiokostas, Christosen_UK
dc.contributor.authorKhan, M G Qen_UK
dc.contributor.authorSelly, Sarah-Louise Cen_UK
dc.contributor.authorHulata, Gideonen_UK
dc.contributor.authorD’Cotta, Helenaen_UK
dc.contributor.authorBaroiller, Jean-Francoisen_UK
dc.contributor.authorMcAndrew, Brendan Jen_UK
dc.contributor.authorPenman, David Jen_UK
dc.date.accessioned2019-09-19T00:00:47Z-
dc.date.available2019-09-19T00:00:47Z-
dc.date.issued2019en_UK
dc.identifier.other13001en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30102-
dc.description.abstractTilapias (family Cichlidae) are of importance in aquaculture and fisheries. Hybridisation and introgression are common within tilapia genera but are difficult to analyse due to limited numbers of species-specific genetic markers. We tested the potential of double digested restriction-site associated DNA (ddRAD) sequencing for discovering single nucleotide polymorphism (SNP) markers to distinguish between 10 tilapia species. Analysis of ddRAD data revealed 1,371 shared SNPs in the de novo-based analysis and 1,204 SNPs in the reference-based analysis. Phylogenetic trees based on these two analyses were very similar. A total of 57 species-specific SNP markers were found among the samples analysed of the 10 tilapia species. Another set of 62 species-specific SNP markers was identified from a subset of four species which have often been involved in hybridisation in aquaculture: 13 for Oreochromis niloticus, 23 for O. aureus, 12 for O. mossambicus and 14 for O. u. hornorum. A panel of 24 SNPs was selected to distinguish among these four species and validated using 91 individuals. Larger numbers of SNP markers were found that could distinguish between the pairs of species within this subset. This technique offers potential for the investigation of hybridisation and introgression among tilapia species in aquaculture and in wild populations.en_UK
dc.language.isoenen_UK
dc.publisherNature Publishing Groupen_UK
dc.relationSyaifudin M, Bekaert M, Taggart JB, Bartie KL, Wehner S, Palaiokostas C, Khan MGQ, Selly SC, Hulata G, D’Cotta H, Baroiller J, McAndrew BJ & Penman DJ (2019) Species-Specific Marker Discovery in Tilapia. Scientific Reports, 9, Art. No.: 13001. https://doi.org/10.1038/s41598-019-48339-2en_UK
dc.rightshis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.en_UK
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/en_UK
dc.titleSpecies-Specific Marker Discovery in Tilapiaen_UK
dc.typeJournal Articleen_UK
dc.identifier.doi10.1038/s41598-019-48339-2en_UK
dc.identifier.pmid31506510en_UK
dc.citation.jtitleScientific Reportsen_UK
dc.citation.issn2045-2322en_UK
dc.citation.volume9en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusVoR - Version of Recorden_UK
dc.citation.date10/09/2019en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationAgricultural Research Organization (ARO)en_UK
dc.contributor.affiliationUniversity of Montpellieren_UK
dc.contributor.affiliationUniversity of Montpellieren_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.isiWOS:000484986300015en_UK
dc.identifier.scopusid2-s2.0-85072019365en_UK
dc.identifier.wtid1447450en_UK
dc.contributor.orcid0000-0002-1206-7654en_UK
dc.contributor.orcid0000-0002-3843-9663en_UK
dc.contributor.orcid0000-0002-3632-2584en_UK
dc.contributor.orcid0000-0002-5948-8800en_UK
dc.contributor.orcid0000-0001-7384-5133en_UK
dc.contributor.orcid0000-0001-8608-6631en_UK
dc.date.accepted2019-07-22en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
dc.date.filedepositdate2019-09-18en_UK
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