Please use this identifier to cite or link to this item: http://hdl.handle.net/1893/30028
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dc.contributor.authorVera, Luisa Men_UK
dc.contributor.authorLock, Erik-Janen_UK
dc.contributor.authorHamre, Kristenen_UK
dc.contributor.authorMigaud, Herveen_UK
dc.contributor.authorLeeming, Danielen_UK
dc.contributor.authorTocher, Douglas Ren_UK
dc.contributor.authorTaylor, John Fen_UK
dc.date.accessioned2019-08-27T09:06:11Z-
dc.date.available2019-08-27T09:06:11Z-
dc.date.issued2019-11en_UK
dc.identifier.other110327en_UK
dc.identifier.urihttp://hdl.handle.net/1893/30028-
dc.description.abstractHighlights The use of plant ingredients affects the level of micronutrients in salmon feeds. Increased micronutrient supplementation reduced the prevalence of spinal deformity. Triploid salmon showed higher prevalence of malformation. In diploids, the expression of bone genes was affected by micronutrient levels. Micronutrients should be supplemented when feeding salmon low marine diets. Previously we showed that, for optimum growth, micronutrient levels should be supplemented above current National Research Council (2011) recommendations for Atlantic salmon when they are fed diets formulated with low levels of marine ingredients. In the present study, the impact of graded levels (100, 200, 400%) of a micronutrient package (NP) on vertebral deformities and bone gene expression were determined in diploid and triploid salmon parr fed low marine diets. The prevalence of radiologically detectable spinal deformities decreased with increasing micronutrient supplementation in both ploidy. On average, triploids had a higher incidence of spinal deformity than diploids within a given diet. Micronutrient supplementation particularly reduced prevalence of fusion deformities in diploids and compression and reduced spacing deformities in triploids. Prevalence of affected vertebrae within each spinal region (cranial, caudal, tail and tail fin) varied significantly between diet and ploidy, and there was interaction. Prevalence of deformities was greatest in the caudal region of triploids and the impact of graded micronutrient supplementation in reducing deformities also greatest in triploids. Diet affected vertebral morphology with length:height (L:H) ratio generally increasing with level of micronutrient supplementation in both ploidy with no difference between ploidy. Increased dietary micronutrients level in diploid salmon increased the vertebral expression of several bone biomarker genes including bone morphogenetic protein 2 (bmp2), osteocalcin (ostcn), alkaline phosphatase (alp), matrix metallopeptidase 13 (mmp13), osteopontin (opn) and insulin-like growth factor 1 receptor (igf1r). In contrast, although some genes showed similar trends in triploids, vertebral gene expression was not significantly affected by dietary micronutrients level. The study confirmed earlier indications that dietary micronutrient levels should be increased in salmon fed diets with low marine ingredients and that there are differences in nutritional requirements between ploidies.en_UK
dc.language.isoenen_UK
dc.publisherElsevieren_UK
dc.relationVera LM, Lock E, Hamre K, Migaud H, Leeming D, Tocher DR & Taylor JF (2019) Enhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploids. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 237, Art. No.: 110327. https://doi.org/10.1016/j.cbpb.2019.110327en_UK
dc.rightsThis item has been embargoed for a period. During the embargo please use the Request a Copy feature at the foot of the Repository record to request a copy directly from the author. You can only request a copy if you wish to use this work for your own research or private study. Accepted refereed manuscript of: Vera LM, Lock E, Hamre K, Migaud H, Leeming D, Tocher DR & Taylor JF (2019) Enhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploids. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology, 237, Art. No.: 110327. DOI: https://doi.org/10.1016/j.cbpb.2019.110327 © 2019, Elsevier. Licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/en_UK
dc.subjectFishmealen_UK
dc.subjectfish oilen_UK
dc.subjectmineralsen_UK
dc.subjectplant proteinsen_UK
dc.subjectploidyen_UK
dc.subjectskeletal deformityen_UK
dc.subjectvegetable oilen_UK
dc.subjectvitaminsen_UK
dc.titleEnhanced micronutrient supplementation in low marine diets reduced vertebral malformation in diploid and triploid Atlantic salmon (Salmo salar) parr, and increased vertebral expression of bone biomarker genes in diploidsen_UK
dc.typeJournal Articleen_UK
dc.rights.embargodate2020-08-26en_UK
dc.rights.embargoreason[Vera-EtAl-CompBiochem_Physiol-2019-Enhanced-micronutrient-supplementation-in-low-marine-diets.pdf] Until this work is published there will be an embargo on the full text of this work. Publisher requires embargo of 12 months after formal publication.en_UK
dc.identifier.doi10.1016/j.cbpb.2019.110327en_UK
dc.citation.jtitleComparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biologyen_UK
dc.citation.issn1879-1107en_UK
dc.citation.issn1096-4959en_UK
dc.citation.volume237en_UK
dc.citation.publicationstatusPublisheden_UK
dc.citation.peerreviewedRefereeden_UK
dc.type.statusAM - Accepted Manuscripten_UK
dc.contributor.funderEuropean Commissionen_UK
dc.author.emailj.f.taylor@stir.ac.uken_UK
dc.citation.date25/08/2019en_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.contributor.affiliationInstitute of Aquacultureen_UK
dc.identifier.wtid1432606en_UK
dc.contributor.orcid0000-0003-0999-055Xen_UK
dc.contributor.orcid0000-0002-5404-7512en_UK
dc.contributor.orcid0000-0002-8603-9410en_UK
dc.contributor.orcid0000-0003-4370-7922en_UK
dc.date.accepted2019-08-22en_UK
dc.description.refREF Compliant by Deposit in Stirling's Repositoryen_UK
dc.date.filedepositdate2019-08-26en_UK
dc.relation.funderprojectARRAINAen_UK
dc.relation.funderrefKBBE-2001-5-288925 ARRAINAen_UK
Appears in Collections:Aquaculture Journal Articles

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